Evidence map›Paper›PMID 36512477›Full record

ArticleStem cells (Dayton, Ohio)2023

ETV2 Upregulation Marks the Specification of Early Cardiomyocytes and Endothelial Cells During Co-differentiation.

Xu Cao, Maria Mircea, Gopala Krishna Yakala, Francijna E van den Hil, Marcella Brescia, Hailiang Mei, Christine L Mummery, Stefan Semrau, Valeria V Orlova

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells (Dayton, Ohio), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.3field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 4 citations in OpenAlex.

  1. Distinct contributions of Etv2Cell reports · 2026
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Xu CaoDepartment of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands.
Maria MirceaLeiden Institute of Physics, Leiden University, Leiden, The Netherlands.
Gopala Krishna YakalaDepartment of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands.
Francijna E van den HilDepartment of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands.
Marcella BresciaDepartment of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands.
Hailiang MeiSequencing Analysis Support Core, Leiden University Medical Center, Leiden, The Netherlands.ORCID 0000-0003-1781-5508
Christine L MummeryDepartment of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands.
Stefan SemrauLeiden Institute of Physics, Leiden University, Leiden, The Netherlands.
Valeria V OrlovaDepartment of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands.ORCID 0000-0002-1169-2802
Leiden University Medical Center · NLLeiden University · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The ability to differentiate human-induced pluripotent stem cells (hiPSCs) efficiently into defined cardiac lineages, such as cardiomyocytes and cardiac endothelial cells, is crucial to study human heart development and model cardiovascular diseases in vitro. The mechanisms underlying the specification of these cell types during human development are not well understood which limits fine-tuning and broader application of cardiac model systems. Here, we used the expression of ETV2, a master regulator of hematoendothelial specification in mice, to identify functionally distinct subpopulations during the co-differentiation of endothelial cells and cardiomyocytes from hiPSCs. Targeted analysis of single-cell RNA-sequencing data revealed differential ETV2 dynamics in the 2 lineages. A newly created fluorescent reporter line allowed us to identify early lineage-predisposed states and show that a transient ETV2-high-state initiates the specification of endothelial cells. We further demonstrated, unexpectedly, that functional cardiomyocytes can originate from progenitors expressing ETV2 at a low level. Our study thus sheds light on the in vitro differentiation dynamics of 2 important cardiac lineages.

Indexed as

Endothelial CellsInduced Pluripotent Stem CellsAnimalsCell DifferentiationEndotheliumHumansMiceMyocytes, CardiacTranscription FactorsUp-RegulationETV2 protein, humanTranscription Factorscardiac differentiationCRISPR/Cas9ETV2ETV2-mCherry fluorescent stem cell reporterhiPSC-derived endothelial cellshuman induced pluripotent stem cells (hiPSCs)RNA sequencingsingle-cell RNA sequencing

Identifiers

PMID36512477
PMCPMC9982073
OpenAlexW4311284209

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.