Evidence map›Paper›PMID 36512242›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Live Imaging of Golgi Outposts in Drosophila Dendritic Arbors.

Josephine W Mitchell, Jill Wildonger

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. The Neuronal Golgi Network and Its Acolytes.Sub-cellular biochemistry · 2026
    Review
  2. Immobilizing Second-InstarCold Spring Harbor protocols · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Josephine W MitchellIntegrated Program in Biochemistry, University of Wisconsin-Madison, Madison, WI, USA.
Jill WildongerPediatrics, University of California, San Diego, La Jolla, CA, USA. jwildonger@health.ucsd.edu.

Funding

MOLECULAR MOTORS AND NEURONAL MICROTUBULE POLARITYR01NS102385 · NINDS · UNIVERSITY OF WISCONSIN-MADISON · PI WILDONGER, JILL C · 2017 to 2022
$1.6M
NINDS NIH HHS R01 NS102385
6 · The paper itself

Abstract

Neurons are polarized cells whose polarity and morphology rely on the robust localization of cellular organelles and cargo to axons or dendrites. Developing neurons require an active secretory pathway, which includes the endoplasmic reticulum and Golgi apparatus, to supply membrane and proteins to growing dendrites and axons. In some neurons, a subset of the Golgi called Golgi "outposts" localize to dendrites and contribute to local secretory networks. The movement and positioning of Golgi outposts have been correlated with dendrite branch growth and stabilization as the dendritic arbor is established. Live imaging is essential to capture the dynamic nature of these organelles. Here we outline a protocol to image and quantify Golgi outposts in peripheral sensory neurons in live, intact Drosophila larvae.

Indexed as

DrosophilaDrosophila ProteinsAnimalsDendritesEndoplasmic ReticulumGolgi ApparatusSensory Receptor CellsDrosophila ProteinsAxonDendriteDrosophilaGolgiGolgi outpostsLive imaging

Identifiers

PMID36512242
PMCPMC11251542

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.