Evidence map›Paper›PMID 36512224›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Common Assays in Mammalian Golgi Studies.

Jie Li, Jianchao Zhang, Sarah Bui, Erpan Ahat, Divya Kolli, Whitney Reid, Lijuan Xing, Yanzhuang Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Jie Li *Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, USA.
Jianchao Zhang *Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, USA.
Sarah Bui *Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, USA.
Erpan AhatDepartment of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, USA.
Divya KolliDepartment of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, USA.
Whitney ReidDepartment of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, USA.
Lijuan XingDepartment of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, USA.
Yanzhuang WangDepartment of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, USA. yzwang@umich.edu.

Funding

Supplement: GOLGI BIOGENESIS AND FUNCTIONR35GM130331 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI WANG, YANZHUANG · 2019 to 2023
$2.7M
Supplement: GOLGI BIOGENESIS AND FUNCTIONR01GM112786 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI WANG, YANZHUANG · 2015 to 2019
$1.3M
NIGMS NIH HHS R01 GM112786NIGMS NIH HHS R35 GM130331
6 · The paper itself

Abstract

The Golgi is a complex structure characterized by stacks of tightly aligned flat cisternae. In mammalian cells, Golgi stacks often concentrate in the perinuclear region and link together to form a ribbon. This structure is dynamic to accommodate continuous cargo flow in and out of the Golgi in both directions and undergoes morphological changes under physiological and pathological conditions. The fine, stacked Golgi structure makes it difficult to study by conventional light or even super-resolution microscopy. Furthermore, efforts to understand how Golgi structural dynamics impact cellular processes have been slow because of the knowledge gap in the protein machinery that maintains the complex and dynamic Golgi structure. In this method article, we list the common assays used in our research to help new and established researchers select the most appropriate method to properly address their questions.

Indexed as

Golgi ApparatusMammalsAnimalsFractionationFunctionGlycosylationGolgi biologyIn vitro techniquesMicroscopyMorphologyProteomicsProximity labelingRNA interferenceRNA-seqSecretomicsTraffickingTransfectionViral system

Identifiers

PMID36512224
PMCPMC10231433

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.