Evidence map›Paper›PMID 36511946›Full record

ArticleChembiochem : a European journal of chemical biology2023

Colorimetric Determination of Adenylation Domain Activity in Nonribosomal Peptide Synthetases by Using Chrome Azurol S.

Lukas Kahlert, Michael S Lichstrahl, Craig A Townsend

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.2field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 9 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Lukas KahlertDepartment of Chemistry, The Johns Hopkins University, 3400 N. Charles Street, Baltimore, Maryland, 21218, USA.ORCID 0000-0002-7549-8603
Michael S LichstrahlDepartment of Chemistry, The Johns Hopkins University, 3400 N. Charles Street, Baltimore, Maryland, 21218, USA.ORCID 0000-0003-1298-4695
Craig A TownsendDepartment of Chemistry, The Johns Hopkins University, 3400 N. Charles Street, Baltimore, Maryland, 21218, USA.ORCID 0000-0003-2795-7585
Johns Hopkins University · US

Funding

Biosynthesis of Beta Lactam AntibioticsR01AI121072 · NIAID · JOHNS HOPKINS UNIVERSITY · PI TOWNSEND, CRAIG ARTHUR · 2016 to 2025
$4.9M
NIAID NIH HHS R01 AI121072
6 · The paper itself

Abstract

Adenylation domains are the main contributor to structural complexity among nonribosomal peptides due to their varied but stringent substrate selection. Several in vitro assays to determine the substrate specificity of these dedicated biocatalysts have been implemented, but high sensitivity is often accompanied by the cost of laborious procedures, expensive reagents or the requirement for auxiliary enzymes. Here, we describe a simple protocol that is based on the removal of ferric iron from a preformed chromogenic complex between ferric iron and Chrome Azurol S. Adenylation activity can be rapidly followed by a decrease in absorbance at 630 nm, visualized by a prominent color change from blue to orange.

Indexed as

ColorimetryPeptide SynthasesHydroxybenzoatesIronSubstrate Specificitychrome azurol SHydroxybenzoatesIronnon-ribosomal peptide synthasePeptide Synthasesadenylation domainsamino acidschromophorescolorimetric assayspyrophosphates

Identifiers

PMID36511946
PMCPMC10041650
OpenAlexW4311286379

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.