ArticleNucleic acids research2022
Targeted bacterial conjugation mediated by synthetic cell-to-cell adhesions.
Article in Nucleic acids research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 37 citations in OpenAlex.
- Engineering rhizobacterial communities for soil and plant health.Microbiology (Reading, England) · 2026Review
- Repurposing the bacterial surface display technology for drug delivery.Advanced drug delivery reviews · 2025Review
- Persistent multispecies dissemination ofJournal of clinical microbiology · 2025Article
- Conjugative delivery of toxin genesJournal of bacteriology · 2025Article
- The extended mobility of plasmids.Nucleic acids research · 2025Review
- Probiotic-mediated tumor microenvironment reprogramming with protease-sensitive interleukin-15 and photothermal therapy.Cell reports. Medicine · 2025Article
- Encounter rates and engagement times limit the transmission of conjugative plasmids.PLoS genetics · 2025Article
- Phage-mediated intercellular CRISPRi for biocomputation in bacterial consortia.Nucleic acids research · 2025Article
- Genetic Bioaugmentation-Mediated Bioremediation of Terephthalate in Soil Microcosms Using an Engineered Environmental Plasmid.Microbial biotechnology · 2025Article
- Diagnosing and engineering gut microbiomes.EMBO molecular medicine · 2024Review
- Design, potential and limitations of conjugation-based antibacterial strategies.Microbial biotechnology · 2024Review
- A whole-cell platform for discovering synthetic cell adhesion molecules in bacteria.Nature communications · 2024Article
- Article
- Emerging methylation-based approaches in microbiome engineering.Biotechnology for biofuels and bioproducts · 2024Review
- Tuning interdomain conjugation to enablemSystems · 2024Article
- Structural and functional diversity of type IV secretion systems.Nature reviews. Microbiology · 2024Review
- Population-level heterogeneity complicates utilization ofmicroPublication biology · 2024Article
- Community context influences the conjugation efficiency ofFEMS microbes · 2024Article
- Article
- Illuminating type IV secretion-mediated DNA trafficking through long filaments.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetic interventions on microbiomes, for clinical or biotechnological purposes, remain challenging. Conjugation-based delivery of genetic cargo is still unspecific and limited by low conjugation rates. Here we report an approach to overcome these problems, based on a synthetic bacterial adhesion system. Mating assemblers consist on a synthetic adhesion formed by the expression on the surface of donor and target cells of specific nanobodies (Nb) and their cognate antigen (Ag). The Nb-Ag bridge increased 1-3 logs transfer of a variety of plasmids, especially in liquid media, confirming that cell-cell docking is a main determinant limiting mating efficiency. Synthetic cell-to-cell adhesion allows efficient conjugation to targeted recipients, enhancing delivery of desired genes to a predefined subset of prey species, or even specific pathogenic strains such as enterohemorrhagic Escherichia coli (EHEC), within a bacterial community. The synthetic conjugation enhancer presented here optimizes plasmid delivery by selecting the target hosts with high selectivity.
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