Evidence map›Paper›PMID 36510221›Full record

ArticleMicrobial cell factories2022

Transcriptome-phenotype matching analysis of how nitrogen sources influence Lacticaseibacillus rhamnosus tolerance to heat stress and oxidative stress.

Chenchen Zhang, Haohao Cheng, Yuemei Han, Yunchao Wa, Dawei Chen, Chengran Guan, Yujun Huang, Ruixia Gu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbial cell factories, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. The Role ofFoods (Basel, Switzerland) · 2025
    Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Chenchen ZhangCollege of Food Science and Engineering, Yangzhou University, 196 Huayang Xilu, Yangzhou, 225100, People's Republic of China.
Haohao ChengCollege of Food Science and Engineering, Yangzhou University, 196 Huayang Xilu, Yangzhou, 225100, People's Republic of China.
Yuemei HanCollege of Food Science and Engineering, Yangzhou University, 196 Huayang Xilu, Yangzhou, 225100, People's Republic of China.
Yunchao WaCollege of Food Science and Engineering, Yangzhou University, 196 Huayang Xilu, Yangzhou, 225100, People's Republic of China.
Dawei ChenCollege of Food Science and Engineering, Yangzhou University, 196 Huayang Xilu, Yangzhou, 225100, People's Republic of China.
Chengran GuanCollege of Food Science and Engineering, Yangzhou University, 196 Huayang Xilu, Yangzhou, 225100, People's Republic of China.
Yujun HuangCollege of Food Science and Engineering, Yangzhou University, 196 Huayang Xilu, Yangzhou, 225100, People's Republic of China.
Ruixia GuCollege of Food Science and Engineering, Yangzhou University, 196 Huayang Xilu, Yangzhou, 225100, People's Republic of China. guruixia1963@163.com.
Yangzhou University · CN

Funding

National Natural Science Foundation of China 31972094National Natural Science Foundation of China 32272333Scientific and Technological Innovation Platform Co-built by Yangzhou City-Yangzhou University YZ2020265
6 · The paper itself

Abstract

backgroundSpray drying is the most cost-effective production method for lactic acid bacteria starters, but heat and oxidative stresses result in low survival rates. The heat stress and oxidative stress tolerance of Lacticaseibacillus rhamnosus cultured in tryptone-free MRS (NP-MRS) broth was much stronger than that in MRS or tryptone-free MRS broth supplemented with phenylalanine (Phe-MRS). Here, multiple transcriptome-phenotype matching was performed on cells cultured in NP-MRS, MRS and Phe-MRS broths to reveal the mechanism by which nitrogen sources influence L. rhamnosus tolerance to heat stress and oxidative stress.

resultsCompared with cells cultured in NP-MRS broth, 83 overlapping differentially expressed genes (DEGs) were downregulated by either tryptone or phenylalanine. The overlapping DEGs were mainly classified into carbohydrate metabolism and membrane transport pathways, which are often repressed by glucose during carbon catabolite repression (CCR). In the presence of glucose, the heat stress or oxidative stress tolerance of L. rhamnosus hsryfm 1301 was not strengthened by supplementation with secondary carbohydrates. Replacing glucose with mannose, fructose or ribose improved the heat stress and oxidative stress tolerance of L. rhamnosus hsryfm 1301 (5 to 46-fold).

conclusionsAlleviation of CCR might be a reason for the resistance of L. rhamnosus hsryfm 1301 to heat stress and oxidative stress in a low-nitrogen environment. The survival rate of L. rhamnosus during spray drying will hopefully be improved by relieving CCR. It is a new discovery that nitrogen sources influence CCR in L. rhamnosus.

Indexed as

Lacticaseibacillus rhamnosusProbioticsGlucoseHeat-Shock ResponseLacticaseibacillusNitrogenOxidative StressPhenylalanineGlucoseNitrogenPhenylalanineCarbon catabolite repressionHeat stressLacticaseibacillus rhamnosusNitrogen sourceOxidative stress

Identifiers

PMID36510221
PMCPMC9746023
OpenAlexW4311244817

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.