Evidence map›Paper›PMID 36510127›Full record

ArticleBMC genomics2022

Regulation of feather follicle development and Msx2 gene SNP degradation in Hungarian white goose.

Yupu Song, Chang Liu, Yuxuan Zhou, Guangyu Lin, Chenguang Xu, Petunia Msuthwana, Sihui Wang, Jingyun Ma, Fangming Zhuang, Xianou Fu and 6 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.9field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 2 institutions in 1 country.

Yupu SongCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China.
Chang LiuChangchun Animal Husbandry Service, Changchun, 130062, China.
Yuxuan ZhouCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China.
Guangyu LinJilin Provincial Animal Husbandry Information Center, Changchun, 130000, China.
Chenguang XuChangchun Animal Husbandry Service, Changchun, 130062, China.
Petunia MsuthwanaCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China.
Sihui WangCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China.
Jingyun MaCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China.
Fangming ZhuangCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China.
Xianou FuCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China.
Yudong WangCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China.
Tuoya LiuCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China.
Qianyan LiuCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China.
Jingbo WangCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China.
Yujian SuiCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China.
Yongfeng SunCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China. sunyongfeng@jlau.edu.cn.
Jilin Agricultural University · CNMinistry of Education of the People's Republic of China · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundHungarian white goose has excellent down production performance and was introduced to China in 2010. The growth and development of feather follicles has an important impact on down production. Goose feather follicles can be divided into primary and secondary feather follicles, both of which originate in the embryonic stage. Msx2 (Msh Homeobox 2) plays a regulatory role in tissues and organs such as eyes, teeth, bones and skin. However, its regulatory mechanism on goose feather follicles development remains unclear.

resultsMsx2 gene first increased, then decreased and increased at the end (E13, E18, E23, E28) during embryonic feather follicle development, and the expression level was the highest at E18. The pEGFP-N1-Msx2 overexpression vector and si-Msx2 siRNA vector were constructed to transfect goose embryo dermal fibroblasts. The results showed that the cell viability of ov-Msx2 group was significantly increased, and the gene expression levels of FGF5 and TGF-β1 genes were significantly down-regulated (P < 0.05), the expressions of PCNA, Bcl2, CDK1, FOXN1 and KGF genes were significantly up-regulated (P < 0.05). After transfection of siRNA vector, the cell viability of the si-Msx2 group was significantly decreased (P < 0.01) compared with the si-NC group. TGF-β1 expression was significantly up-regulated (P < 0.05), FGF5 expression was extremely significantly up-regulated (P < 0.01), while PCNA, Bcl2, CDK1, FOXN1 and KGF gene expression was significantly down-regulated (P < 0.05). High-throughput sequencing technology was used to mine the exon SNPs of Msx2. A total of 11 SNP loci were screened, four of the SNPs located in exon 1 were missense mutations. The feather follicle diameter of the GC genotype at the G78C site is significantly larger than that of the other two genotypes.

conclusionsMsx2 maybe inhibit the apoptosis of goose dermal fibroblasts and promotes their proliferation. G78C can be used as a potential molecular marker for downy Variety.

Indexed as

GeeseTransforming Growth Factor beta1AnimalsEmbryonic DevelopmentFeathersProto-Oncogene Proteins c-bcl-2Proto-Oncogene Proteins c-bcl-2Transforming Growth Factor beta1Feather follicleGoose downHungarian white gooseSNP

Identifiers

PMID36510127
PMCPMC9743523
OpenAlexW4311574977

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.