ArticlePloS one2022
Development of a recombinase polymerase amplification assay with lateral flow dipstick (RPA-LFD) for rapid detection of Shigella spp. and enteroinvasive Escherichia coli.
Article in PloS one, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- Coupling Exponential Rolling Circle Amplification to Lateral Flow Devices for Point-of-Care Detection of Viral RNA.Angewandte Chemie (International ed. in English) · 2026Article
- Development and Application of a Dual-Readout RPA-PfAgo System for Rapid Detection ofVeterinary sciences · 2026Article
- Rapid visual detection ofOpen veterinary journal · 2026Article
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- Polymerase Chain Reaction-Lateral Flow Strip for DetectingAntibiotics (Basel, Switzerland) · 2025Article
- Technologies for detecting biological risk factors in agricultural products and their applications.Current research in food science · 2025Review
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- An RPA-Based CRISPR/Cas12a Assay in Combination with a Lateral Flow Assay for the Rapid Detection ofFoods (Basel, Switzerland) · 2024Article
- Recombinase-aid amplification combined with lateral flow detection assay for sex identification of the great white pelican (Pelecanus onocrotalus).Scientific reports · 2024Article
- The Tomato Variety Affects the Survival ofPathogens (Basel, Switzerland) · 2024Article
- Recombinase Polymerase Amplification Combined with Lateral Flow Dipstick Assay for the Rapid and Sensitive Detection ofInternational journal of molecular sciences · 2024Article
- A novel detection method based on MIRA-CRISPR/Cas13a-LFD targeting the repeated DNA sequence of Trichomonas vaginalis.Parasites & vectors · 2024Article
- Rapid on-site detection of echinococcosis and schistosomiasis based on RPA.Memorias do Instituto Oswaldo Cruz · 2024Article
- A New Duplex Recombinase Polymerase Amplification (D-RPA) Method for the Simultaneous and Rapid Detection ofFoods (Basel, Switzerland) · 2023Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Shigella spp. and enteroinvasive Escherichia coli (EIEC) are widely distributed and can cause serious food-borne diseases for humans such as dysentery. Therefore, an efficient detection platform is needed to detect Shigella and EIEC quickly and sensitively. In this study, a method called recombinase polymerase amplification combined with lateral flow dipstick (RPA-LFD) was developed for rapid detection of Shigella and EIEC. RPA primers and LFD detection probes were designed for their shared virulence gene ipaH. Primers and probes were screened, and the primer concentration, and reaction time and temperature were optimized. According to the optimization results, the RPA reaction should be performed at 39°C, and when combined with LFD, it takes less than 25 min for detection with the naked eye. The developed RPA-LFD method specifically targets gene ipaH and has no cross-reactivity with other common food-borne pathogens. In addition, the minimum detection limit of RPA-LFD is 1.29×102 copies/μL. The detection of food sample showed that the RPA-LFD method was also verified for the detection of actual samples.
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Registered trials
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