Evidence map›Paper›PMID 36507958›Full record

ArticleAnalytical and bioanalytical chemistry2023

Development of an improved standard reference material for folate vitamers in human serum.

Johanna E Camara, Jeanita S Pritchett, Yasmine C Daniels, Mary Bedner, Michael A Nelson, Mark S Lowenthal, Zia Fazili, Christine M Pfeiffer, Karen W Phinney, Katherine E Sharpless and 5 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical and bioanalytical chemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 5 institutions in 1 country.

Johanna E CamaraNational Institute of Standards and Technology, Gaithersburg, MD, USA. johanna.camara@nist.gov.
Jeanita S PritchettNational Institute of Standards and Technology, Gaithersburg, MD, USA.
Yasmine C DanielsNational Institute of Standards and Technology, Gaithersburg, MD, USA.
Mary BednerNational Institute of Standards and Technology, Gaithersburg, MD, USA.
Michael A NelsonNational Institute of Standards and Technology, Gaithersburg, MD, USA.
Mark S LowenthalNational Institute of Standards and Technology, Gaithersburg, MD, USA.
Zia FaziliCenters for Disease Control and Prevention, National Center for Environmental Health, Atlanta, GA, USA.
Christine M PfeifferCenters for Disease Control and Prevention, National Center for Environmental Health, Atlanta, GA, USA.
Karen W PhinneyNational Institute of Standards and Technology, Gaithersburg, MD, USA.
Katherine E SharplessNational Institute of Standards and Technology, Gaithersburg, MD, USA.
Lane C SanderNational Institute of Standards and Technology, Gaithersburg, MD, USA.
Katrice A LippaNational Institute of Standards and Technology, Gaithersburg, MD, USA.
James H YenNational Institute of Standards and Technology, Gaithersburg, MD, USA.
Adam J KuszakOffice of Dietary Supplements, National Institutes of Health, Bethesda, MD, USA.
Stephen A WiseOffice of Dietary Supplements, National Institutes of Health, Bethesda, MD, USA.
National Institute of Standards and Technology · USCenters for Disease Control and Prevention · USNational Center for Environmental Health · USNational Institutes of Health · USOffice of Dietary Supplements · US

Funding

Intramural CDC HHS CC999999
6 · The paper itself

Abstract

The US National Institute of Standards and Technology (NIST) developed a Standard Reference Material® (SRM®) 3949 Folate Vitamers in Frozen Human Serum to replace SRM 1955 Homocysteine and Folate in Human Serum. The presence of increased endogenous levels of folic acid and 5-methyltetrahydrofolate (5mTHF) in SRM 3949, enhanced folate stability via addition of ascorbic acid, and inclusion of values for additional minor folates are improvements over SRM 1955 that should better serve the clinical folate measurement community. The new SRM contains folates at three levels. To produce SRM 3949, pilot sera were collected from 15 individual donors, 5 of whom were given a 400-µg folic acid supplement 1 h prior to blood draw to increase serum levels of 5mTHF and folic acid for the high-level material. To stabilize the folates, 0.5% (mass concentration) ascorbic acid was added as soon as possible after preparation of serum. These pilot sera were screened for five folates plus the pyrazino-s-triazine derivative of 4-α-hydroxy-5-methyltetrahydrofolate (MeFox) at the US Centers for Disease Control and Prevention (CDC) by isotope dilution liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS). Based on these results, a blending protocol was specified to obtain the three desired folate concentrations for SRM 3949. ID-LC-MS/MS analysis at the CDC and NIST was utilized to assign values for folic acid and 5mTHF, as well as several minor folates.

Indexed as

Folic AcidTandem Mass SpectrometryAscorbic AcidChromatography, LiquidHumansReference StandardsAscorbic AcidFolic AcidFolateHuman serumLiquid chromatography-tandem mass spectrometryReference material

Identifiers

PMID36507958
PMCPMC11284738
OpenAlexW4311205510

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.