ArticleNano letters2023
Orientation-Locked DNA Origami for Stable Trapping of Small Proteins in the Nanopore Electro-Osmotic Trap.
Article in Nano letters, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Solid-state nanopore sensing: the next workhorse of biophysical characterization.Nature methods · 2026Review
- Denoise Stepwise Signals by Diffusion Model-Based Approach.Small methods · 2026Article
- A programmable DNA origami nanosyringe for directed membrane translocation.Nature nanotechnology · 2026Article
- Single-Molecule Detection Concepts Enabled by DNA Origami.Micromachines · 2026Review
- DNA Origami and Their Application in Biosensors.Biosensors · 2026Review
- Review
- Nanopore Trap for Label-Free Fingerprinting of Surface-modified Single Nanoparticles.Small methods · 2026Article
- Selective and Controllable Trapping of Single Proteins in Nanopores Using Reversible Covalent Bonds.ACS nano · 2026Article
- Engineering DNA nanopores: from structural evolution to sensing and transport.Materials today. Bio · 2025Review
- The Electric Field in Solid State Nanopores Causes Dissociation of Strong Biomolecular Interactions.Nano letters · 2025Article
- DNA Origami Incorporated into Solid-State Nanopores Enables Enhanced Sensitivity for Precise Analysis of Protein Translocations.Analytical chemistry · 2024Article
- Understanding Electrophoresis and Electroosmosis in Nanopore Sensing with the Help of the Nanopore Electro-Osmotic Trap.ACS nano · 2024Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nanopores are versatile single-molecule sensors offering a simple label-free readout with great sensitivity. We recently introduced the nanopore electro-osmotic trap (NEOtrap) which can trap and sense single unmodified proteins for long times. The trapping is achieved by the electro-osmotic flow (EOF) generated from a DNA-origami sphere docked onto the pore, but thermal fluctuations of the origami limited the trapping of small proteins. Here, we use site-specific cholesterol functionalization of the origami sphere to firmly link it to the lipid-coated nanopore. We can lock the origami in either a vertical or horizontal orientation which strongly modulates the EOF. The optimized EOF greatly enhances the trapping capacity, yielding reduced noise, reduced measurement heterogeneity, an increased capture rate, and 100-fold extended observation times. We demonstrate the trapping of a variety of single proteins, including small ones down to 14 kDa. The cholesterol functionalization significantly expands the application range of the NEOtrap technology.
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