Development of a microwave-based extraction for forensic biological samples.

Fabiana Taglia, Ling Wang, Casandra H Setser, Nicole Fernández-Tejero, Bruce R McCord, Steven B Lee

Open access · goldAbstract read

In one paragraph

Article in Forensic science international. Synergy, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

0.2field-weighted citation impact, top 56% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Fabiana TagliaDepartment of Chemistry and Biochemistry, Florida International University, Miami, FL, USA.

Ling WangDepartment of Chemistry and Biochemistry, Florida International University, Miami, FL, USA.

Casandra H SetserDepartment of Chemistry and Biochemistry, Florida International University, Miami, FL, USA.

Nicole Fernández-TejeroDepartment of Chemistry and Biochemistry, Florida International University, Miami, FL, USA.

Bruce R McCordDepartment of Chemistry and Biochemistry, Florida International University, Miami, FL, USA.

Steven B LeeDepartment of Chemistry and Biochemistry, Florida International University, Miami, FL, USA.

Florida International University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this study, a quick microwave-based treatment was developed as a front end for DNA analysis of forensic samples. The effect of microwave treatment is to cause cell disruption which can improve the release of DNA during direct PCR as well as with extraction methods. Exposure to microwave preprocessing improved the quality of rapid genotyping, particularly when used with low level samples. Optimal results were obtained when samples were microwaved at 300W for 40 s, resulting in improved allele detection. Overall, the addition of this simple preprocessing step improves sensitivity and allele recovery for low level DNA samples when combined with expedited DNA analysis workflows. Its main advantages include speed, low cost, compatibility with downstream DNA methods and application to a wide variety of samples.

Indexed as

Direct PCRForensic analysisMicrowaveMicrowave extractionRapid DNASaliva

Identifiers

PMID36506483

DOI10.1016/j.fsisyn.2022.100291

PMCPMC9731879

OpenAlexW4310816243

What OpenQuestion holds

Textmetadata

LicenceCC BY-NC-ND

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.