ArticleFrontiers in veterinary science2022
Measurement of fecal T3 metabolite levels in sheep: Analytical and biological validation of the method.
Article in Frontiers in veterinary science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 3 citations in OpenAlex.
- Metabolic, Hormonal and Body Condition Changes in Melatonin-Implanted Dairy Rams and Flock Pregnancy Rate During the Mating Season: A Longitudinal Field Study.Animals : an open access journal from MDPI · 2026Article
- Can Fecal T3 Metabolite Level Fluctuations in European Roe Deer (Capreolus capreolus) Give Insights on Body Condition and Thermal Stress?Integrative zoology · 2026Article
- Validation of a triiodothyronine (T3) ELISA for mouse fecal samples.Physiological reports · 2024Article
- Fecal thyroid hormone metabolites in wild ungulates: a mini-review.Frontiers in veterinary science · 2024Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Introduction: Biological sample collection from wild and farms animals is often associated with difficulties related to the handling and restraint procedures, and most of the time it could induce stress, altering the welfare and physiological homeostasis. The analysis of fecal T3 metabolites (FTMs) allows to test samples collected in a non-invasive manner, providing several information about the animal's physiological conditions and the effects related to environmental and nutritional variations. This procedure has found wide application in wild species, but less in domestic ones. Methods: The aim of this work was to validate the use of an immuno-enzymatic competitive ELISA kit, designed for T3 quantification in human blood serum samples, for the assessment of FTMs in the sheep. For the analytical validation, precision, recovery and parallelism were evaluated; for biological validation the variations of FTMs in relation to age, sex and the physiological status of the animal were determined. Results: After a verification of the precision (RSD % < 15%), mean recovery (75%) and parallelism (CV% < 10%), the kit was used to measure FTMs in cyclic, pregnant, and early lactating ewes as well as in rams and ewe lambs. The results showed that FTMs concentrations in pregnant ewes were significantly lower ( Conclusion: In conclusion the present study demonstrates that FTMs can be reliably and accurately determined in sheep feces, using an ELISA kit formulated for human serum T3 assay. The application of this method in the livestock sector could allow to improve our knowledge about the response of animals to different physiological and environmental conditions, and thus assess their welfare.
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