ArticleMicrobiome2022
Targeted single-cell genomics reveals novel host adaptation strategies of the symbiotic bacteria Endozoicomonas in Acropora tenuis coral.
Article in Microbiome, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 34 citations in OpenAlex.
- CoSAG-nf: a scalable nextflow pipeline for co-assembly, optimization, and interactive visualization of high-throughput single-cell genomes.Bioinformatics (Oxford, England) · 2026Article
- Endozoicomonas acroporae enhances coral thermal resilience through host-microbe coordination.The ISME journal · 2026Article
- Resolving the evolutionary duality of marine symbionts: redefining the genusISME communications · 2026Article
- Subcuticular symbionts of intertidal brittle stars: diversity, host specificity, and functional potential.ISME communications · 2026Article
- Genomic prediction of symbiotic interactions between two Endozoicomonas clades and their coral host, Acropora loripes.Animal microbiome · 2025Article
- Article
- Bacterial Dynamics in Newly Settled Acropora kenti: Insights From Inoculations With Individual Probiotic Candidates.Environmental microbiology · 2025Article
- Jellyfish blooms through the microbial lens: temporal changes, cross-species and Jellyfish-water comparisons.Environmental microbiome · 2025Article
- Genomic insights into hostMicrobial genomics · 2025Article
- Metagenomic Characterization of Microbiome Taxa Associated with Coral Reef Communities in North Area of Tabuk Region, Saudia Arabia.Life (Basel, Switzerland) · 2025Article
- Evidence for microbially-mediated tradeoffs between growth and defense throughout coral evolution.Animal microbiome · 2025Article
- Differential aggregation patterns of Endozoicomonas within tissues of the coral Acropora loripes.The ISME journal · 2025Article
- GenomeFISH: genome-based fluorescence in situ hybridization for strain-level visualization of microbial communities.The ISME journal · 2025Article
- Single-cell genomics of single soil aggregates: methodological assessment and potential implications with a focus on nitrogen metabolism.Frontiers in microbiology · 2025Article
- The coral microbiome in sickness, in health and in a changing world.Nature reviews. Microbiology · 2024Review
- Tools for microbial single-cell genomics for obtaining uncultured microbial genomes.Biophysical reviews · 2024Review
- Uncultured prokaryotic genomes in the spotlight: An examination of publicly available data from metagenomics and single-cell genomics.Computational and structural biotechnology journal · 2023Review
- Massively parallel single-cell genomics of microbiomes in rice paddies.Frontiers in microbiology · 2022Article
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Authors and funding
16 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundEndozoicomonas bacteria symbiosis with various marine organisms is hypothesized as a potential indicator of health in corals. Although many amplicon analyses using 16S rRNA gene have suggested the diversity of Endozoicomonas species, genome analysis has been limited due to contamination of host-derived sequences and difficulties in culture and metagenomic analysis. Therefore, the evolutionary and functional potential of individual Endozoicomonas species symbiotic with the same coral species remains unresolved.
resultsIn this study, we applied a novel single-cell genomics technique using droplet microfluidics to obtain single-cell amplified genomes (SAGs) for uncultured coral-associated Endozoicomonas spp. We obtained seven novel Endozoicomonas genomes and quantitative bacterial composition from Acropora tenuis corals at four sites in Japan. Our quantitative 16S rRNA gene and comparative genomic analysis revealed that these Endozoicomonas spp. belong to different lineages (Clade A and Clade B), with widely varying abundance among individual corals. Furthermore, each Endozoicomonas species possessed various eukaryotic-like genes in clade-specific genes. It was suggested that these eukaryotic-like genes might have a potential ability of different functions in each clade, such as infection of the host coral or suppression of host immune pathways. These Endozoicomonas species may have adopted different host adaptation strategies despite living symbiotically on the same coral.
conclusionsThis study suggests that coral-associated Endozoicomonas spp. on the same species of coral have different evolutional strategies and functional potentials in each species and emphasizes the need to analyze the genome of each uncultured strain in future coral-Endozoicomonas relationships studies. Video Abstract.
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