Evidence map›Paper›PMID 36503524›Full record

ArticleBMC veterinary research2022

Transcriptome analysis of PK-15 cells expressing CSFV NS4A.

Huifang Lv, Zhifeng Peng, Bingxin Jia, Huiyuan Jing, Sufang Cao, Zhikun Xu, Wang Dong

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Strategies of Classical Swine Fever Immune Evasion.International journal of molecular sciences · 2025
    Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Huifang LvKey Laboratory of Veterinary Biological Products, College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, 450046, Zhengzhou, China.
Zhifeng PengKey Laboratory of Veterinary Biological Products, College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, 450046, Zhengzhou, China.
Bingxin JiaKey Laboratory of Veterinary Biological Products, College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, 450046, Zhengzhou, China.
Huiyuan JingKey Laboratory of Veterinary Biological Products, College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, 450046, Zhengzhou, China.
Sufang CaoKey Laboratory of Veterinary Biological Products, College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, 450046, Zhengzhou, China.
Zhikun XuKey Laboratory of Veterinary Biological Products, College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, 450046, Zhengzhou, China.
Wang DongKey Laboratory of Veterinary Biological Products, College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, 450046, Zhengzhou, China. superdong1990@163.com.
Henan University · CNHenan Agricultural University · CN

Funding

Doctoral Science Foundation of Henan University of Animal Husbandry and Economy 2019HNUAHEDF019Doctoral Science Foundation of Henan University of Animal Husbandry and Economy 2019HNUAHEDF020Innovation Team of Henan University of Animal Husbandry and Economy 2018KYTD16Key Disciplines of Veterinary Medicine of Henan University of Animal Husbandry and Economy XJXK202202Key Scientific Research Project of University in Henan Province 20A230005Key Scientific Research Project of University in Henan Province 21B230004Key Technologies Research and Development Program of Henan Province 222102110312National Natural Science Foundation of China 31902246National Natural Science Foundation of China 31902274
6 · The paper itself

Abstract

backgroundClassical swine fever (CSF) is a severe disease of pigs that results in huge economic losses worldwide and is caused by classical swine fever virus (CSFV). CSFV nonstructural protein 4 A (NS4A) plays a crucial role in infectious CSFV particle formation. However, the function of NS4A during CSFV infection is not well understood. 

resultsIn this study, we used RNA-seq to investigate the functional role of CSFV NS4A in PK-15 cells. A total of 3893 differentially expressed genes (DEGs) were identified in PK-15 cells expressing NS4A compared to cells expressing the empty vector (NC). Twelve DEGs were selected and further verified by RT‒qPCR. GO and KEGG enrichment analyses revealed that these DEGs were associated with multiple biological functions, including cell adhesion, apoptosis, host defence response, the inflammatory response, the immune response, and autophagy. Interestingly, some genes associated with host immune defence and inflammatory response were downregulated, and some genes associated with host apoptosis and autophagy were upregulated.

conclusionCSFV NS4A inhibits the innate immune response, and suppresses the expression of important genes associated with defence response to viruses and inflammatory response, and regulates cell adhesion, apoptosis and autophagy.

Indexed as

Classical Swine FeverClassical Swine Fever VirusSwine DiseasesAnimalsCell LineGene Expression ProfilingSwineVirus ReplicationClassical swine fever virusImmune defenceNS4ARNA-seqVirus replication

Identifiers

PMID36503524
PMCPMC9742017
OpenAlexW4311678949

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.