Evidence map›Paper›PMID 36499395›Full record

ArticleInternational journal of molecular sciences2022

A Rapid and Specific Real-Time PCR Assay for the Detection of Clinically Relevant Mucorales Species.

Massimiliano Bergallo, Vivian Tullio, Janira Roana, Valeria Allizond, Narcisa Mandras, Valentina Daprà, Maddalena Dini, Sara Comini, Lorenza Cavallo, Stefano Gambarino and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.8field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 1 institution in 1 country.

Massimiliano BergalloCytoimmunodiagnostic Laboratory, Department of Public Health and Pediatrics, University of Turin, Piazza Polonia 94, 10126 Turin, Italy.
Vivian TullioBacteriology and Mycology Laboratory, Department of Public Health and Pediatrics, University of Turin, Piazza Polonia 94, 10126 Turin, Italy.ORCID 0000-0002-8660-4248
Janira RoanaBacteriology and Mycology Laboratory, Department of Public Health and Pediatrics, University of Turin, Piazza Polonia 94, 10126 Turin, Italy.ORCID 0000-0003-2542-622X
Valeria AllizondBacteriology and Mycology Laboratory, Department of Public Health and Pediatrics, University of Turin, Piazza Polonia 94, 10126 Turin, Italy.ORCID 0000-0003-0585-9247
Narcisa MandrasBacteriology and Mycology Laboratory, Department of Public Health and Pediatrics, University of Turin, Piazza Polonia 94, 10126 Turin, Italy.ORCID 0000-0002-2949-4556
Valentina DapràCytoimmunodiagnostic Laboratory, Department of Public Health and Pediatrics, University of Turin, Piazza Polonia 94, 10126 Turin, Italy.
Maddalena DiniCytoimmunodiagnostic Laboratory, Department of Public Health and Pediatrics, University of Turin, Piazza Polonia 94, 10126 Turin, Italy.
Sara CominiBacteriology and Mycology Laboratory, Department of Public Health and Pediatrics, University of Turin, Piazza Polonia 94, 10126 Turin, Italy.
Lorenza CavalloBacteriology and Mycology Laboratory, Department of Public Health and Pediatrics, University of Turin, Piazza Polonia 94, 10126 Turin, Italy.ORCID 0000-0002-8701-8486
Stefano GambarinoCytoimmunodiagnostic Laboratory, Department of Public Health and Pediatrics, University of Turin, Piazza Polonia 94, 10126 Turin, Italy.
Anna Maria CuffiniBacteriology and Mycology Laboratory, Department of Public Health and Pediatrics, University of Turin, Piazza Polonia 94, 10126 Turin, Italy.
Giuliana BancheBacteriology and Mycology Laboratory, Department of Public Health and Pediatrics, University of Turin, Piazza Polonia 94, 10126 Turin, Italy.ORCID 0000-0003-2219-0223
University of Turin · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Infections triggered by filamentous fungi placed in the order Mucorales, phylum Zygomycota, can cause serious harm to immunocompromised patients. Since there is lack of a standardized PCR (polymerase chain reaction) assay for early diagnosis of this fungal infection, this work was aimed to develop a new PCR assay able to detect the presence of Mucorales genera in clinical specimens. Here, we describe a novel diagnostic TaqMan MGB probe assay for precise and rapid detection of the most common clinical species of Mucorales. Zygomycete-specific oligonucleotides were designed to specifically amplify and bind highly conserved sequences of fungal 28S rRNA gene. Additionally, we succeeded in differentiating Mucorales species (i.e.,

Indexed as

MucoralesMucormycosisHumansImmunocompromised HostReal-Time Polymerase Chain ReactionRNA, Ribosomal, 28SRNA, Ribosomal, 28Sdiagnostic microbiologyfilamentous fungiMucoralesmucormycosisprimer and probe designTaqMan MGB assay

Identifiers

PMID36499395
PMCPMC9735628
OpenAlexW4311050768

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.