ArticleInternational journal of molecular sciences2022
An Improved Real-Time Viability PCR Assay to Detect
Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The trial behind it
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Who cites it
9 citing papers in PubMed.
- Development and Validation of a Viability RT-qPCR Assay for Detecting Infectious Spring Viraemia of Carp Virus (SVCV).Journal of fish diseases · 2026Article
- Biosensing technologies for foodborne pathogen detection and healthcare: principles, emerging materials, and intelligent platforms.Mikrochimica acta · 2026Review
- Optimized Viability-ddPCR with Triton X-100 Enhancement for Selective Detection of LiveJournal of microbiology and biotechnology · 2026Article
- Rapid differentiation of viable and inactivated African swine fever virus by a viability quantitative PCR.Veterinary research · 2025Article
- Bacterial gastroenteritis in the world of culture-independent diagnostic testing: a study to evaluate the kinetics of bacterial shedding by culture and CIDT.Microbiology spectrum · 2025Observational
- Propidium Monoazide is Unreliable for Quantitative Live-Dead Molecular Assays.Analytical chemistry · 2025Article
- A challenging STEC strain isolation from patients' stools: an O166:H15 STEC strain with theMicrobiology spectrum · 2024Article
- Assessing probiotic viability in mixed species yogurt using a novel propidium monoazide (PMAxx)-quantitative PCR method.Frontiers in microbiology · 2024Article
- A Pilot Study to Detect ViableInternational journal of molecular sciences · 2023Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Viability PCR (vPCR) uses a DNA intercalating dye to irreversibly bind double-stranded DNA from organisms with compromised cell membranes. This allows the selective amplification of DNA from intact cells. An optimized vPCR protocol should minimize false positives (DNA from compromised cells not fully removed) and false negatives (live cell DNA bound by the dye). We aimed to optimize a vPCR protocol using PMAxx™ as the intercalating agent and
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Registered trials
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