Evidence map›Paper›PMID 36496813›Full record

ArticleAnimals : an open access journal from MDPI2022

Microsatellite Analysis Revealed Potential DNA Markers for Gestation Length and Sub-Population Diversity in Kari Sheep.

Muhammad Ibrahim, Sohail Ahmad, Israr Ud Din, Waqas Ahmad, Ijaz Ahmad, Sher Hayat Khan, Ihtesham Ul Haq, Jehan Zeb, Olivier Andre Sparagano

Open access · goldAbstract read
In one paragraph

Article in Animals : an open access journal from MDPI, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.9field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Muhammad IbrahimInstitute of Biotechnology and Genetic Engineering, The University of Agriculture Peshawar, Peshawar 25130, Pakistan.
Sohail AhmadInstitute of Biotechnology and Genetic Engineering, The University of Agriculture Peshawar, Peshawar 25130, Pakistan.
Israr Ud DinInstitute of Biotechnology and Genetic Engineering, The University of Agriculture Peshawar, Peshawar 25130, Pakistan.ORCID 0000-0002-0643-2408
Waqas AhmadInstitute of Biotechnology and Genetic Engineering, The University of Agriculture Peshawar, Peshawar 25130, Pakistan.
Ijaz AhmadCollege of Veterinary Sciences, The University of Agriculture Peshawar, Peshawar 25130, Pakistan.
Sher Hayat KhanInstitute of Biotechnology and Genetic Engineering, The University of Agriculture Peshawar, Peshawar 25130, Pakistan.
Ihtesham Ul HaqInstitute of Biotechnology and Genetic Engineering, The University of Agriculture Peshawar, Peshawar 25130, Pakistan.
Jehan ZebDepartment of Infectious Diseases and Public Health, City University of Hong Kong, Hong Kong SAR, China.
Olivier Andre SparaganoDepartment of Infectious Diseases and Public Health, City University of Hong Kong, Hong Kong SAR, China.ORCID 0000-0003-3141-310X
The University of Agriculture, Peshawar · PKCity University of Hong Kong · HK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Kari sheep inhabiting the Chitral district of Pakistan show variation in gestation length. In this study, we have analyzed the genetic differences between the three subtypes of Kari sheep (based on variation in gestation length) using microsatellite markers. Kari sheep samples were collected from their breeding tract and were characterized for gestation length and genetic diversity using microsatellite markers. A total of 78 Kari ewes were grouped into three categories based on gestation length (GL), i.e., Kari-S (with a shorter GL), Kari-M (with a medium GL), and Kari-L (with a longer GL). DNA from these samples was used to amplify 31 ovine-specific microsatellite loci through PCR. Of the total 78 Kari specimens, 24 were grouped in Kari-S (GL = 100.7 ± 1.8), 26 were from the Kari-M subtype (GL = 123.1 ± 1.0), and 28 were Kari-L (GL = 143.8 ± 1.5). Microsatellite analysis revealed an association of genotypes at two marker sites (MAF214 and ILSTS5) with variation in GL. A total of 158 alleles were detected across the 22 polymorphic loci with an average of 7.18 alleles per locus. Unique alleles were found in all three subtypes. The highest number of unique alleles was observed in Kari-L (15), followed by Kari-S (10) and Kari-M (8). The results indicated that Kari-S is a genetically distinct subtype (with higher genetic differentiation and distance) from Kari-M and Kari-L. The genetic uniqueness of Kari-S is important for further exploration of the genetic basis for shorter gestation length, and exploitation of their unique values.

Indexed as

bottleneck analysisgenetic diversitygestation lengthKari sheepmicrosatellite association

Identifiers

PMID36496813
PMCPMC9736151
OpenAlexW4310193561

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.