ArticleScience advances2022
Robust induction of primordial germ cells of white rhinoceros on the brink of extinction.
Article in Science advances, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 54 citations in OpenAlex.
- De-extinction technology and its application to conservation.The Journal of heredity · 2026Review
- Review
- Induction of pluripotency in large Japanese field mouse (Apodemus speciosus) fibroblasts via reprogramming.The Journal of veterinary medical science · 2026Article
- Article
- Reconstitution of germ cell and gonadal development for in vitro gamete production.The Journal of reproduction and development · 2026Review
- De-extinction of the Northern white rhinoceros (Ceratotherium simum cottoni).The Journal of reproduction and development · 2026Review
- Generation and transcriptome profiling of bonobo induced pluripotent stem cells using stealth RNA vectors: a tripartite comparative study with humans and chimpanzees.BMC genomics · 2025Article
- Establishment, Characterization, and Cryopreservation of Feather Follicle Fibroblast Lines From Hyacinth Macaw (Anodorhynchus hyacinthinus).Cell biology international · 2025Article
- Application of induced pluripotent stem cells in the conservation of endangered animals.Stem cell research & therapy · 2025Review
- Genomic map of the functionally extinct northern white rhinoceros (Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- What do women and rhinos have in common?F&S reports · 2025Article
- Outside the box: comparative stem cell insights for fertility biotechnology and conservation.F&S reports · 2025Article
- High levels of histone acetylation modifications promote the formation of PGCs.Poultry science · 2025Article
- Can stem cells save the animals?Nature methods · 2025Article
- Advancing stem cell technologies for conservation of wildlife biodiversity.Development (Cambridge, England) · 2024Review
- Generation and characterization of giant panda induced pluripotent stem cells.Science advances · 2024Article
- Mechanisms of Embryonic Stem Cell Pluripotency Maintenance and Their Application in Livestock and Poultry Breeding.Animals : an open access journal from MDPI · 2024Review
- A Standardized Nomenclature Design for Systematic Referencing and Identification of Animal Cellular Material.Animals : an open access journal from MDPI · 2024Article
- Emerging Contributions of Pluripotent Stem Cells to Reproductive Technologies in Veterinary Medicine.Journal of developmental biology · 2024Review
- In Vitro Maturation, In Vitro Oogenesis, and Ovarian Longevity.Reproductive sciences (Thousand Oaks, Calif.) · 2024Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 6 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vitro gametogenesis, the process of generating gametes from pluripotent cells in culture, is a powerful tool for improving our understanding of germ cell development and an alternative source of gametes. Here, we induced primordial germ cell-like cells (PGCLCs) from pluripotent stem cells of the northern white rhinoceros (NWR), a species for which only two females remain, and southern white rhinoceros (SWR), the closest species to the NWR. PGCLC differentiation from SWR embryonic stem cells is highly reliant on bone morphogenetic protein and WNT signals. Genetic analysis revealed that SRY-box transcription factor 17 (SOX17) is essential for SWR-PGCLC induction. Under the defined condition, NWR induced pluripotent stem cells differentiated into PGCLCs. We also identified cell surface markers, CD9 and Integrin subunit alpha 6 (ITGA6), that enabled us to isolate PGCLCs without genetic alteration in pluripotent stem cells. This study provides a first step toward the production of NWR gametes in culture and understanding of the basic mechanism of primordial germ cell specification in a large animal.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.