Evidence map›Paper›PMID 36484723›Full record

ArticleAnalytical chemistry2022

"Organ-in-a-Column" Coupled On-line with Liquid Chromatography-Mass Spectrometry.

Stian Kogler, Aleksandra Aizenshtadt, Sean Harrison, Frøydis Sved Skottvoll, Henriette Engen Berg, Shadab Abadpour, Hanne Scholz, Gareth Sullivan, Bernd Thiede, Elsa Lundanes and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical chemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Article
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  3. Review
  4. Review
  5. Article
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  8. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Stian KoglerHybrid Technology Hub─Centre of Excellence, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, P.O. Box 1110, Blindern, 0317 Oslo, Norway.ORCID 0000-0002-5366-8958
Aleksandra AizenshtadtHybrid Technology Hub─Centre of Excellence, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, P.O. Box 1110, Blindern, 0317 Oslo, Norway.
Sean HarrisonHybrid Technology Hub─Centre of Excellence, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, P.O. Box 1110, Blindern, 0317 Oslo, Norway.ORCID 0000-0001-9399-9631
Frøydis Sved SkottvollHybrid Technology Hub─Centre of Excellence, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, P.O. Box 1110, Blindern, 0317 Oslo, Norway.
Henriette Engen BergSection for Chemical Life Sciences, Department of Chemistry, University of Oslo, P.O. Box 1033, Blindern, 0315 Oslo, Norway.
Shadab AbadpourHybrid Technology Hub─Centre of Excellence, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, P.O. Box 1110, Blindern, 0317 Oslo, Norway.
Hanne ScholzHybrid Technology Hub─Centre of Excellence, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, P.O. Box 1110, Blindern, 0317 Oslo, Norway.
Gareth SullivanDepartment of Pediatric Research, Oslo University Hospital, P.O. Box 4950, Nydalen, 0424 Oslo, Norway.
Bernd ThiedeSection for Biochemistry and Molecular Biology, Department of Biosciences, University of Oslo, P.O. Box 1066, Blindern, 0316 Oslo, Norway.ORCID 0000-0002-2804-9522
Elsa LundanesSection for Chemical Life Sciences, Department of Chemistry, University of Oslo, P.O. Box 1033, Blindern, 0315 Oslo, Norway.
Inger Lise BogenSection for Drug Abuse Research, Department of Forensic Sciences, Oslo University Hospital, P.O. Box 4950, Nydalen, 0424 Oslo, Norway.
Stefan KraussHybrid Technology Hub─Centre of Excellence, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, P.O. Box 1110, Blindern, 0317 Oslo, Norway.
Hanne Røberg-LarsenSection for Chemical Life Sciences, Department of Chemistry, University of Oslo, P.O. Box 1033, Blindern, 0315 Oslo, Norway.
Steven Ray WilsonHybrid Technology Hub─Centre of Excellence, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, P.O. Box 1110, Blindern, 0317 Oslo, Norway.ORCID 0000-0002-9755-1188

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Organoids, i.e., laboratory-grown organ models developed from stem cells, are emerging tools for studying organ physiology, disease modeling, and drug development. On-line analysis of organoids with mass spectrometry would provide analytical versatility and automation. To achieve these features with robust hardware, we have loaded liquid chromatography column housings with induced pluripotent stem cell (iPSC) derived liver organoids and coupled the "organ-in-a-column" units on-line with liquid chromatography-mass spectrometry (LC-MS). Liver organoids were coloaded with glass beads to achieve an even distribution of organoids throughout the column while preventing clogging. The liver organoids were interrogated "on column" with heroin, followed by on-line monitoring of the drug's phase 1 metabolism. Enzymatic metabolism of heroin produced in the "organ-in-a-column" units was detected and monitored using a triple quadrupole MS instrument, serving as a proof-of-concept for on-line coupling of liver organoids and mass spectrometry. Taken together, the technology allows direct integration of liver organoids with LC-MS, allowing selective and automated tracking of drug metabolism over time.

Indexed as

HeroinLiverAutomationChromatography, LiquidMass SpectrometryHeroin

Identifiers

PMID36484723
PMCPMC9773175

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.