Evidence map›Paper›PMID 36482332›Full record

ArticleBMC microbiology2022

Transcriptome analysis and cytochrome P450 monooxygenase reveal the molecular mechanism of Bisphenol A degradation by Pseudomonas putida strain YC-AE1.

Adel Eltoukhy, Yang Jia, Imane Lamraoui, M A Abo-Kadoum, Omar Mohammad Atta, Ruth Nahurira, Junhuan Wang, Yanchun Yan

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
3.8field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 26 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 5 institutions in 4 countries.

Adel EltoukhyBotany and Microbiology Department, Faculty of Science, Al-Azhar University, Assiut, 71524, Egypt. adelaly@azhar.edu.eg.
Yang JiaNational and Local Joint Engineering Research Center of Ecological Treatment Technology for Urban Water Pollution, and Zhejiang Provincial Key Lab for Subtropical Water Environment and Marine Biological Resources Protection, Wenzhou University, Wenzhou, 325035, China.
Imane LamraouiHigher National School of Biotechnology "Toufik Khaznadar" (ENSB), 25000, Constantine, Algeria.
M A Abo-KadoumBotany and Microbiology Department, Faculty of Science, Al-Azhar University, Assiut, 71524, Egypt.
Omar Mohammad AttaBotany and Microbiology Department, Faculty of Science, Al-Azhar University, Assiut, 71524, Egypt.
Ruth NahuriraFaculty of Science, Kabale University, Kabale, Uganda.
Junhuan WangGraduate School of Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Yanchun YanGraduate School of Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Al-Azhar University · EGChinese Academy of Agricultural Sciences · CNHigher National Veterinary School · DZKabale University · UGWenzhou University · CN

Funding

Basic Research Fund of Chinese Academy of Agricultural Sciences 1610042017001Basic Research Fund of Chinese Academy of Agricultural Sciences 1610042018005Basic Research Fund of Chinese Academy of Agricultural Sciences 1610042018006National Natural Science Foundation of China 21876201National Natural Science Foundation of China 31540067
6 · The paper itself

Abstract

backgroundBisphenol A (BPA) is a rapid spreading organic pollutant that widely used in many industries especially as a plasticizer in polycarbonate plastic and epoxy resins. BPA reported as a prominent endocrine disruptor compound that possesses estrogenic activity and fulminant toxicity. Pseudomonas putida YC-AE1 was isolated in our previous study and exerted a strong degradation capacity toward BPA at high concentrations; however, the molecular degradation mechanism is still enigmatic.

resultsWe employed RNA sequencing to analyze the differentially expressed genes (DEGs) in the YC-AE1 strain upon BPA induction. Out of 1229 differentially expressed genes, 725 genes were positively regulated, and 504 genes were down-regulated. The pathways of microbial metabolism in diverse environments were significantly enriched among DEGs based on KEGG enrichment analysis. qRT-PCR confirm the involvement of BPA degradation relevant genes in accordance with RNA Seq data. The degradation pathway of BPA in YC-AE1 was proposed with specific enzymes and encoded genes. The role of cytochrome P450 (CYP450) in BPA degradation was further verified. Sever decrease in BPA degradation was recorded by YC-AE1 in the presence of CYP450 inhibitor. Subsequently, CYP450bisdB deficient YC-AE1 strain △ bisdB lost its ability toward BPA transformation comparing with the wild type. Furthermore, Transformation of E. coli with pET-32a-bisdAB empowers it to degrade 66 mg l

conclusionIn this study we propose the molecular basis and the potential role of YC-AE1cytochrome P450 monooxygenase in BPA catabolism.

Indexed as

Benzhydryl CompoundsCytochrome P-450 Enzyme SystemPhenolsPseudomonas putidaBisphenol A CompoundsGene Expression ProfilingBenzhydryl Compoundsbisphenol ABisphenol A CompoundsCytochrome P-450 Enzyme SystemPhenolsBisphenol ACytochrome P450Degradation pathwayPseudomonas putida YC-AE1RNA sequencing

Identifiers

PMID36482332
PMCPMC9733184
OpenAlexW4311668562

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.