Evidence map›Paper›PMID 36482295›Full record

ArticleMicrobial cell factories2022

Advanced one-pot deconstruction and valorization of lignocellulosic biomass into triacetic acid lactone using Rhodosporidium toruloides.

Peter B Otoupal, Gina M Geiselman, Asun M Oka, Carolina A Barcelos, Hemant Choudhary, Duy Dinh, Wenqing Zhong, HeeJin Hwang, Jay D Keasling, Aindrila Mukhopadhyay and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbial cell factories, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.2field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 4 institutions in 3 countries.

Peter B OtoupalBiomanufacturing and Biomaterials Department, Sandia National Laboratories, Livermore, CA, USA.
Gina M GeiselmanBiomanufacturing and Biomaterials Department, Sandia National Laboratories, Livermore, CA, USA.
Asun M OkaAdvanced Biofuels and Bioproducts Process Development Unit, Lawrence Berkeley National Laboratory, Emeryville, CA, USA.
Carolina A BarcelosAdvanced Biofuels and Bioproducts Process Development Unit, Lawrence Berkeley National Laboratory, Emeryville, CA, USA.
Hemant ChoudharyBiomanufacturing and Biomaterials Department, Sandia National Laboratories, Livermore, CA, USA.
Duy DinhAdvanced Biofuels and Bioproducts Process Development Unit, Lawrence Berkeley National Laboratory, Emeryville, CA, USA.
Wenqing ZhongAdvanced Biofuels and Bioproducts Process Development Unit, Lawrence Berkeley National Laboratory, Emeryville, CA, USA.
HeeJin HwangBiomanufacturing and Biomaterials Department, Sandia National Laboratories, Livermore, CA, USA.
Jay D KeaslingDOE Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, CA, USA.
Aindrila MukhopadhyayBiomanufacturing and Biomaterials Department, Sandia National Laboratories, Livermore, CA, USA.
Eric SundstromAdvanced Biofuels and Bioproducts Process Development Unit, Lawrence Berkeley National Laboratory, Emeryville, CA, USA.
Robert W HaushalterDOE Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, CA, USA.
Ning SunAdvanced Biofuels and Bioproducts Process Development Unit, Lawrence Berkeley National Laboratory, Emeryville, CA, USA.
Blake A SimmonsDOE Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, CA, USA.
John M GladdenBiomanufacturing and Biomaterials Department, Sandia National Laboratories, Livermore, CA, USA. jmgladden@lbl.gov.
Lawrence Berkeley National Laboratory · USJoint BioEnergy Institute · USSandia National Laboratories California · USTechnical University of Denmark · DK

Funding

Biological and Environmental Research DE-AC02-05CH11231
6 · The paper itself

Abstract

backgroundRhodosporidium toruloides is capable of co-utilization of complex carbon sources and robust growth from lignocellulosic hydrolysates. This oleaginous yeast is therefore an attractive host for heterologous production of valuable bioproducts at high titers from low-cost, deconstructed biomass in an economically and environmentally sustainable manner. Here we demonstrate this by engineering R. toruloides to produce the polyketide triacetic acid lactone (TAL) directly from unfiltered hydrolysate deconstructed from biomass with minimal unit process operations.

resultsIntroduction of the 2-pyrone synthase gene into R. toruloides enabled the organism to produce 2.4 g/L TAL from simple media or 2.0 g/L from hydrolysate produced from sorghum biomass. Both of these titers are on par with titers from other better-studied microbial hosts after they had been heavily engineered. We next demonstrate that filtered hydrolysates produced from ensiled sorghum are superior to those derived from dried sorghum for TAL production, likely due to the substantial organic acids produced during ensiling. We also demonstrate that the organic acids found in ensiled biomass can be used for direct synthesis of ionic liquids within the biomass pretreatment process, enabling consolidation of unit operations of in-situ ionic liquid synthesis, pretreatment, saccharification, and fermentation into a one-pot, separations-free process. Finally, we demonstrate this consolidation in a 2 L bioreactor using unfiltered hydrolysate, producing 3.9 g/L TAL.

conclusionMany steps involved in deconstructing biomass into fermentable substrate can be combined into a distinct operation, and directly fed to cultures of engineered R. toruloides cultures for subsequent valorization into gram per liter titers of TAL in a cost-effective manner.

Indexed as

LactonesLigninPyronesRhodotorulaBiomassFermentationHydrolysisMetabolic EngineeringSorghumLactonesLigninlignocellulosePyronestriacetic acid lactone

Identifiers

PMID36482295
PMCPMC9733078
OpenAlexW4311007080

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.