Evidence map›Paper›PMID 36478869›Full record

ArticleFrontiers in microbiology2022

Construction of nucleus-directed fluorescent reporter systems and its application to verification of heterokaryon formation in

Qianqian Zhang, Fang Shu, Xin Chen, Wei Liu, Yinbing Bian, Heng Kang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Large-Scale Field Cultivation ofJournal of fungi (Basel, Switzerland) · 2023
    Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Qianqian ZhangInstitute of Applied Mycology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Fang ShuInstitute of Applied Mycology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Xin ChenInstitute of Applied Mycology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Wei LiuInstitute of Applied Mycology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Yinbing BianInstitute of Applied Mycology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Heng KangInstitute of Applied Mycology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Methods: Considering this, we constructed pEH2B and pMH2B vectors by fusing Results: The fluorescence observation and molecular identification results indicated the successful hyphal fusion and heterokaryon formation. We found that the expression of the reporter genes was stable, and it did not interfere with the growth of the fungus. Discussion: Our constructed nucleus-directed fluorescent systems in

Indexed as

Agrobacterium-mediated transformationfluorescent proteinheterokaryon formationhistone H2BMorchella importuna

Identifiers

PMID36478869
PMCPMC9720127

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.