ArticlePlant biotechnology journal2023
Profiling of phytohormone-specific microRNAs and characterization of the miR160-ARF1 module involved in glandular trichome development and artemisinin biosynthesis in Artemisia annua.
Article in Plant biotechnology journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 42 citations in OpenAlex.
- A Systematic Review of Alternative Artemisinin Production Strategies.International journal of molecular sciences · 2025Pooled it
- Envisaging the role of plant Small RNAs: A comprehensive review on antiviral defense mechanisms.Molecular biology reports · 2026Review
- ArtemisiaDB: A comprehensive multi-omics database for Artemisia annua.Plant communications · 2026Article
- Non-coding RNA: a new perspective on the regulation of secondary metabolites in medicinal plants.Molecular horticulture · 2026Review
- Regulatory architecture of high-altitude adaptation in Artemisia: from signal perception to specialized metabolism.Plant cell reports · 2026Review
- Integrative chromosome-level genomics and metabolomics uncover regulatory networks linking monoterpenoid biosynthesis and glandular trichome formation inHorticulture research · 2026Article
- Integrative Roles of miRNAs and circRNAs in Plant Antiviral Gene Regulation and Autophagy.Plants (Basel, Switzerland) · 2025Review
- Interacting MeZFP29 and MebZIPW regulates MeNRT2.2 from cassava responding to nitrate signaling.Plant cell reports · 2025Article
- OsMAINTENANCE OF MERISTEM LIKE 1 controls style number at high temperatures in rice.Plant molecular biology · 2025Article
- Fine-tuning plant valuable secondary metabolite biosynthesis via small RNA manipulation: strategies and potential.Planta · 2024Review
- TrichomeLess Regulator 3 is required for trichome initial and cuticle biosynthesis in Artemisia annua.Molecular horticulture · 2024Article
- Graphene enhances artemisinin production in the traditional medicinal plant Artemisia annua via dynamic physiological processes and miRNA regulation.Plant communications · 2024Article
- A non-canonicalFrontiers in plant science · 2024Article
- Article
- Applying quantitative spatial phenotypes analysis to the investigation of peltate glandular trichomes development pattern in Perilla frutescens.Plant methods · 2023Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs) play crucial roles in plant development and secondary metabolism through different modes of sequence-specific interaction with their targets. Artemisinin biosynthesis is extensively regulated by phytohormones. However, the function of phytohormone-responsive miRNAs in artemisinin biosynthesis remains enigmatic. Thus, we combined the analysis of transcriptomics, small RNAs, and the degradome to generate a comprehensive resource for identifying key miRNA-target circuits involved in the phytohormone-induced process of artemisinin biosynthesis in Artemisia annua. In total, 151 conserved and 52 novel miRNAs and their 4132 targets were determined. Based on the differential expression analysis, miR160 was selected as a potential miRNA involved in artemisinin synthesis. Overexpressing MIR160 significantly impaired glandular trichome formation and suppressed artemisinin biosynthesis in A. annua, while repressing its expression resulted in the opposite effect, indicating that miR160 negatively regulates glandular trichome development and artemisinin biosynthesis. RNA ligase-mediated 5' RACE and transient transformation assays showed that miR160 mediates the RNA cleavage of Auxin Response Factor 1 (ARF1) in A. annua. Furthermore, ARF1 was shown to increase artemisinin synthesis by activating AaDBR2 expression. Taken together, our results reveal the intrinsic link between the miR160-ARF1 module and artemisinin biosynthesis, and may expedite the innovation of metabolic engineering approaches for high and stable production of artemisinin in the future.
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