ArticleNucleic acids research2023
Hierarchical DNA branch assembly-encoded fluorescent nanoladders for single-cell transcripts imaging.
Article in Nucleic acids research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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3 citing papers in PubMed, 16 citations in OpenAlex.
- A general and scalable DNA nano-chip with a fully localized architecture enables biocomputing in living cells and precisely induces cell apoptosis.Chemical science · 2026Article
- Dephosphorylation-regulated MNAzyme-PER cascade as a programmable converter for multi-task molecular actuation.Journal of nanobiotechnology · 2026Article
- Visualizing epigenetic modifications and their spatial proximities in single cells using three DNA-encoded amplifying FISH imaging strategies: BEA-FISH, PPDA-FISH and Cell-TALKING.Nature protocols · 2025Article
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Authors and funding
6 authors at 1 institution in 1 country.
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Abstract
Spatial visualization of single-cell transcripts is limited by signal specificity and multiplexing. Here, we report hierarchical DNA branch assembly-encoded fluorescent nanoladders, which achieve denoised and highly multiplexed signal amplification for single-molecule transcript imaging. This method first offers independent RNA-primed rolling circle amplification without nonspecific amplification based on circular DNAzyme. It then executes programmable DNA branch assembly on these amplicons to encode virtual signals for visualizing numbers of targets by FISH. In theory, more virtual signals can be encoded via the increase of detection spectral channels and repeats of the same sequences on barcode. Our method almost eliminates nonspecific amplification in fixed cells (reducing nonspecific spots of single cells from 16 to nearly zero), and achieves simultaneous quantitation of nine transcripts by using only two detection spectral channels. We demonstrate accurate RNA profiling in different cancer cells, and reveal diverse localization patterns for spatial regulation of transcripts.
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