Evidence map›Paper›PMID 36477983›Full record

ArticleJournal of medical entomology2023

Identification of MicroRNAs in the West Nile Virus Vector Culex tarsalis (Diptera: Culicidae).

Sultan Asad, Ahmed M Mehdi, Sujit Pujhari, Claudia Rückert, Gregory D Ebel, Jason L Rasgon

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of medical entomology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Sultan AsadDepartment of Entomology, The Center for Infectious Disease Dynamics, and the Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, USA.
Ahmed M MehdiThe University of Queensland, Brisbane, Australia Diamantina Institute, Faculty of Medicine, The University of Queensland, Translational Research Institute, Brisbane, QLD, Australia.
Sujit PujhariDepartment of Entomology, The Center for Infectious Disease Dynamics, and the Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, USA.
Claudia RückertDepartment of Biochemistry and Molecular Biology, University of Nevada Reno, Reno, NV, 89557, USA.
Gregory D EbelDepartment of Microbiology, Immunology and Pathology, Center for Vector-borne Infectious Diseases, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, 80523, USAand.
Jason L RasgonDepartment of Entomology, The Center for Infectious Disease Dynamics, and the Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, USA.ORCID 0000-0002-4050-8429

Funding

Hologenomic basis of WNV vector competence in Culex tarsalisR01AI150251 · NIAID · PENNSYLVANIA STATE UNIVERSITY, THE · PI RASGON, JASON L · 2019 to 2024
$2.7M
Somatic and germline gene transduction in mosquitoesR01AI128201 · NIAID · PENNSYLVANIA STATE UNIVERSITY, THE · PI Jason L Rasgon · 2017 to 2026
$2.4M
Wolbachia-induced enhancement of human arboviral pathogensR01AI116636 · NIAID · PENNSYLVANIA STATE UNIVERSITY, THE · PI RASGON, JASON L · 2016 to 2020
$1.9M
NIAID NIH HHS R01 AI116636NIAID NIH HHS R01 AI128201NIAID NIH HHS R01 AI150251NIH HHS R01AI150251
6 · The paper itself

Abstract

MicroRNAs (miRNAs) are a group of small noncoding RNAs that regulate gene expression during important biological processes including development and pathogen defense in most living organisms. Presently, no miRNAs have been identified in the mosquito Culex tarsalis (Diptera: Culicidae), one of the most important vectors of West Nile virus (WNV) in North America. We used small RNA sequencing data and in vitro and in vivo experiments to identify and validate a repertoire of miRNAs in Cx. tarsalis mosquitoes. Using bioinformatic approaches we analyzed small RNA sequences from the Cx. tarsalis CT embryonic cell line to discover orthologs for 86 miRNAs. Consistent with other mosquitoes such as Aedes albopictus and Culex quinquefasciatus, miR-184 was found to be the most abundant miRNA in Cx. tarsalis. We also identified 20 novel miRNAs from the recently sequenced Cx. tarsalis genome, for a total of 106 miRNAs identified in this study. The presence of selected miRNAs was biologically validated in both the CT cell line and in adult Cx. tarsalis mosquitoes using RT-qPCR and sequencing. These results will open new avenues of research into the role of miRNAs in Cx. tarsalis biology, including development, metabolism, immunity, and pathogen infection.

Indexed as

AedesCulexCulicidaeMicroRNAsWest Nile FeverWest Nile virusAnimalsMosquito VectorsMicroRNAsblood feedingCulex tarsalismicroRNAs

Identifiers

PMID36477983
PMCPMC10216877

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.