ArticleNucleic acids research2023
ONE-seq: epitranscriptome and gene-specific profiling of NAD-capped RNA.
Article in Nucleic acids research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- Emerging strategies and innovations in circular RNA synthesis.Molecular therapy. Nucleic acids · 2026Review
- Discovery of 5' NAD capped viral RNAs reveals evolutionary divergent 5' metabolite capping across hepaciviruses.Nature communications · 2026Article
- High-resolution mapping reveals features of bacterial NAD-capped RNAs and stress-responsive transcription initiation.Nature communications · 2026Article
- Quantification and transcriptome profiling reveal abundant, dynamic and translatable dephospho-CoA-capped RNAs.Nature biotechnology · 2026Article
- CompasSeq: epitranscriptome-wide percentage assessment of metabolite-capped RNA at the transcript resolution.Nature communications · 2025Article
- The Mysterious World of Non-Canonical Caps - What We Know and Why We Need New Sequencing Techniques.Chembiochem : a European journal of chemical biology · 2025Review
- HIV-1 Infection Reduces NAD Capping of Host Cell snRNA and snoRNA.ACS chemical biology · 2024Article
- Identification and in vitro characterization of UDP-GlcNAc-RNA cap-modifying and decapping enzymes.Nucleic acids research · 2024Article
- Hidden features of NAD-RNA epitranscriptome iniScience · 2024Article
- If the 5' cap fits (wear it) - Non-canonical RNA capping.RNA biology · 2024Review
- Epitranscriptome analysis of NAD-capped RNA by spike-in-based normalization and prediction of chronological age.iScience · 2023Article
- Impact of NAD+ metabolism on ovarian aging.Immunity & ageing : I & A · 2023Review
- Identification of NAD-RNA species and ADPR-RNA decapping in Archaea.Nature communications · 2023Article
- Future Perspectives for the Identification and Sequencing of Nicotinamide Adenine Dinucleotide-Capped RNAs.Accounts of chemical research · 2023Article
- NADcapPro and circNC: methods for accurate profiling of NAD and non-canonical RNA caps in eukaryotes.Communications biology · 2023Article
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Authors and funding
16 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The hub metabolite, nicotinamide adenine dinucleotide (NAD), can be used as an initiating nucleotide in RNA synthesis to result in NAD-capped RNAs (NAD-RNA). Since NAD has been heightened as one of the most essential modulators in aging and various age-related diseases, its attachment to RNA might indicate a yet-to-be discovered mechanism that impacts adult life-course. However, the unknown identity of NAD-linked RNAs in adult and aging tissues has hindered functional studies. Here, we introduce ONE-seq method to identify the RNA transcripts that contain NAD cap. ONE-seq has been optimized to use only one-step chemo-enzymatic biotinylation, followed by streptavidin capture and the nudix phosphohydrolase NudC-catalyzed elution, to specifically recover NAD-capped RNAs for epitranscriptome and gene-specific analyses. Using ONE-seq, we discover more than a thousand of previously unknown NAD-RNAs in the mouse liver and reveal epitranscriptome-wide dynamics of NAD-RNAs with age. ONE-seq empowers the identification of NAD-capped RNAs that are responsive to distinct physiological states, facilitating functional investigation into this modification.
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