ArticleNucleic acids research2022
The mechanisms of siRNA selection by plant Argonaute proteins triggering DNA methylation.
Article in Nucleic acids research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- Decoding Argonaute specificity: insights fromRNA (New York, N.Y.) · 2026Review
- Exogenous 24nt siRNAs induce AGO4A-dependent silencing via promoter DNA methylation and H3K9me2 deposition.Frontiers in plant science · 2026Article
- Regulatory Landscapes of Non-Coding RNAs During Drought Stress in Plants.International journal of molecular sciences · 2025Review
- Redox-Epigenetic Crosstalk in Plant Stress Responses: The Roles of Reactive Oxygen and Nitrogen Species in Modulating Chromatin Dynamics.International journal of molecular sciences · 2025Review
- Small Interfering RNAs as Critical Regulators of Plant Life Process: New Perspectives on Regulating the Transcriptomic Machinery.International journal of molecular sciences · 2025Review
- Proteolytic control of the RNA silencing machinery.The Plant cell · 2024Review
- The Biosynthesis Process of Small RNA and Its Pivotal Roles in Plant Development.International journal of molecular sciences · 2024Review
- The clade-specific target recognition mechanisms of plant RISCs.Nucleic acids research · 2024Article
- Barley AGO4 proteins show overlapping functionality with distinct small RNA-binding properties in heterologous complementation.Plant cell reports · 2024Article
- Emerging Roles of Epigenetics in Grapevine and Winegrowing.Plants (Basel, Switzerland) · 2024Review
- HRDE-2 drives small RNA specificity for the nuclear Argonaute protein HRDE-1.Nature communications · 2024Article
- The plant siRNA landscape.The Plant cell · 2024Review
- Beyond Loading: Functions of Plant ARGONAUTE Proteins.International journal of molecular sciences · 2023Review
- A Non-Canonical Pathway Induced by Externally Applied Virus-Specific dsRNA in Potato Plants.International journal of molecular sciences · 2023Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The model plant Arabidopsis thaliana encodes as many as ten Argonaute proteins (AGO1-10) with different functions. Each AGO selectively loads a set of small RNAs by recognizing their length and 5' nucleotide identity to properly regulate target genes. Previous studies showed that AGO4 and AGO6, key factors in DNA methylation, incorporate 24-nt small-interfering RNAs with 5' adenine (24A siRNAs). However, it has been unclear how these AGOs specifically load 24A siRNAs. Here, we biochemically investigated the siRNA preference of AGO4, AGO6 and their chimeric mutants. We found that AGO4 and AGO6 use distinct mechanisms to preferentially load 24A siRNAs. Moreover, we showed that the 5' A specificity of AGO4 and AGO6 is not determined by the previously known nucleotide specificity loop in the MID domain but rather by the coordination of the MID and PIWI domains. These findings advance our mechanistic understanding of how small RNAs are accurately sorted into different AGO proteins in plants.
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