ArticleRegenerative therapy2022
Transplantation of chemically-induced liver progenitor cells ameliorates hepatic fibrosis in mice with diet-induced nonalcoholic steatohepatitis.
Article in Regenerative therapy, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 10 citations in OpenAlex.
- Efficacy of xeno-free induced mesenchymal stem/stromal cells in enhancing autologous fat graft survival.Breast cancer (Tokyo, Japan) · 2026Article
- Integration of transplanted chemically induced liver progenitor cells into recipient bile ducts in a rat bile duct transection model.PloS one · 2026Article
- Small Extracellular Vesicles Released From Human Chemically Induced Liver Progenitors Have the Potential to Improve Liver Fibrosis in Mice.Gastro hep advances · 2026Review
- Transplantation of chemically induced liver progenitors in Nagase analbuminemic rats under liver regenerative stimulus.Regenerative therapy · 2025Article
- Development of rematuration of canine chemically induced hepatic progenitor cells using 3D rocking culture.Regenerative therapy · 2025Article
- Reprogramming canine cryopreserved hepatocytes to hepatic progenitor cells using small molecule compounds.Regenerative therapy · 2024Article
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15 authors at 2 institutions in 1 country.
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Abstract
Chemically-induced liver progenitors (CLiPs) have promising applications in liver regenerative medicine. We aimed to clarify the efficacy of CLiPs for ameliorating fibrosis in a diet-induced nonalcoholic steatohepatitis rat model, since nonalcoholic fatty liver disease is currently recognized as the most common form of chronic liver disease in developed countries.
methodsPrimary mature hepatocytes were isolated from 7-week-old male Wistar rats. To establish CLiPs, isolated hepatocytes were cultured in differentiation medium composed of Y-27632, A-83-01, and CHIR99021 (YAC medium). As an animal model that reproduces NASH pathophysiology, 6-week-old severe combined immunodeficient (SCID) mice were carefully selected and prepared and fed with choline-deficient, L-amino acid-defined, high-fat diet (HFD). After 12 weeks' HFD feeding, the mice were assigned to continue HFD with or without the administration of rat CLiPs (HFD + CLiPs and HFD-CLiPs, respectively). Rat CLiPs were administered from the spleen. Hepatic fibrosis was semi-quantitatively evaluated according to histology. Liver parenchyma and blood samples were collected for biochemical analyses.
resultsRat CLiPs were positive for CK19 and EpCAM were successfully delivered to the liver. At 8 weeks after CLiPs transplantation, the HFD + CLiPs group showed significantly less positive staining than the HFD-CLiPs group. Alanine aminotransferase significantly improved in the HFD + CLiPs group, as demonstrated by Azan staining and αSMA immunostaining. RT qPCR showed that the liver expression of MMP2 and 9 tended to be higher in the HFD + CLiPs group.
conclusionsThe anti-fibrotic effect of CLiPs was demonstrated in the immunodeficient NASH animal model and may have therapeutic applications in humans.
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