ArticleThe Plant cell2023
Producing fast and active Rubisco in tobacco to enhance photosynthesis.
Article in The Plant cell, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 55 citations in OpenAlex.
- PtCP1 Is an Extraplastidial Cysteine Protease Involved in Leaf Protein Degradation ofPlants (Basel, Switzerland) · 2026Article
- Editing and synthetic engineering of chloroplast genomes.Plant communications · 2026Review
- Not just Rubisco: integrated strategies to overcome the biochemical limitations of CFrontiers in plant science · 2026Review
- Advancements in photosynthetic efficiency: Pathways, regulation, and biotechnological applications for enhancing crop productivity.Plant signaling & behavior · 2025Review
- Effects of Nanomaterials on Crops.Current issues in molecular biology · 2025Review
- Engineering COInternational journal of molecular sciences · 2025Article
- Replacement of large subunit N terminus enabled biogenesis of different plant Rubiscos in E. coli.Plant biotechnology journal · 2025Article
- Understanding carboxysomes to enhance carbon fixation in crops.Biochemical Society transactions · 2025Review
- Engineering Rubisco condensation in chloroplasts to manipulate plant photosynthesis.Plant biotechnology journal · 2025Article
- Engineering COPlant communications · 2025Article
- Genetic engineering of RuBisCO by multiplex CRISPR editing small subunits in rice.Plant biotechnology journal · 2025Article
- Discovery of diverse chimeric peptides in a eukaryotic proteome sets the stage for experimental validation of the mosaic translation hypothesis.Computational and structural biotechnology journal · 2025Article
- Comparative Insights into Photosynthetic, Biochemical, and Ultrastructural Mechanisms in Hibiscus and Pelargonium Plants.Plants (Basel, Switzerland) · 2024Article
- Genome-Wide Analysis and Expression Profiling of SoybeanInternational journal of molecular sciences · 2024Article
- From leaf to multiscale models of photosynthesis: applications and challenges for crop improvement.Photosynthesis research · 2024Review
- Improving Crop Yield through Increasing Carbon Gain and Reducing Carbon Loss.Plants (Basel, Switzerland) · 2024Review
- Molecular characterization and expression pattern of Rubisco activase gene GhRCAβ2 in upland cotton (Gossypium hirsutum L.).Genes & genomics · 2024Article
- Knocking Out Chloroplastic Aldolases/Rubisco Lysine Methyltransferase Enhances Biomass Accumulation inInternational journal of molecular sciences · 2024Article
- Engineering Rubisco to enhance COSynthetic and systems biotechnology · 2024Review
- Decreased Photosynthetic Efficiency inPlants (Basel, Switzerland) · 2024Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
Abstract
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) performs most of the carbon fixation on Earth. However, plant Rubisco is an intrinsically inefficient enzyme given its low carboxylation rate, representing a major limitation to photosynthesis. Replacing endogenous plant Rubisco with a faster Rubisco is anticipated to enhance crop photosynthesis and productivity. However, the requirement of chaperones for Rubisco expression and assembly has obstructed the efficient production of functional foreign Rubisco in chloroplasts. Here, we report the engineering of a Form 1A Rubisco from the proteobacterium Halothiobacillus neapolitanus in Escherichia coli and tobacco (Nicotiana tabacum) chloroplasts without any cognate chaperones. The native tobacco gene encoding Rubisco large subunit was genetically replaced with H. neapolitanus Rubisco (HnRubisco) large and small subunit genes. We show that HnRubisco subunits can form functional L8S8 hexadecamers in tobacco chloroplasts at high efficiency, accounting for ∼40% of the wild-type tobacco Rubisco content. The chloroplast-expressed HnRubisco displayed a ∼2-fold greater carboxylation rate and supported a similar autotrophic growth rate of transgenic plants to that of wild-type in air supplemented with 1% CO2. This study represents a step toward the engineering of a fast and highly active Rubisco in chloroplasts to improve crop photosynthesis and growth.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.