Evidence map›Paper›PMID 36471561›Full record

ArticleChembiochem : a European journal of chemical biology2023

Facile de Novo Sequencing of Tetrazine-Cyclized Peptides through UV-Induced Ring-Opening and Cleavage from the Solid Phase.

Ariane Borges, Chi Nguyen, Madison Letendre, Iryna Onasenko, Rene Kandler, Ngoc K Nguyen, Jue Chen, Tamara Allakhverdova, Emily Atkinson, Bella DiChiara and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Ariane BorgesCarlson School of Chemistry and Biochemistry, Clark University, 950 Main Street, 01610, Worcester, MA, USA.
Chi NguyenCarlson School of Chemistry and Biochemistry, Clark University, 950 Main Street, 01610, Worcester, MA, USA.
Madison LetendreCarlson School of Chemistry and Biochemistry, Clark University, 950 Main Street, 01610, Worcester, MA, USA.
Iryna OnasenkoCarlson School of Chemistry and Biochemistry, Clark University, 950 Main Street, 01610, Worcester, MA, USA.
Rene KandlerCarlson School of Chemistry and Biochemistry, Clark University, 950 Main Street, 01610, Worcester, MA, USA.
Ngoc K NguyenCarlson School of Chemistry and Biochemistry, Clark University, 950 Main Street, 01610, Worcester, MA, USA.
Jue ChenCarlson School of Chemistry and Biochemistry, Clark University, 950 Main Street, 01610, Worcester, MA, USA.
Tamara AllakhverdovaCarlson School of Chemistry and Biochemistry, Clark University, 950 Main Street, 01610, Worcester, MA, USA.
Emily AtkinsonCarlson School of Chemistry and Biochemistry, Clark University, 950 Main Street, 01610, Worcester, MA, USA.
Bella DiChiaraCarlson School of Chemistry and Biochemistry, Clark University, 950 Main Street, 01610, Worcester, MA, USA.
Caroline WangCarlson School of Chemistry and Biochemistry, Clark University, 950 Main Street, 01610, Worcester, MA, USA.
Noa PetlerCarlson School of Chemistry and Biochemistry, Clark University, 950 Main Street, 01610, Worcester, MA, USA.
Henna PatelCarlson School of Chemistry and Biochemistry, Clark University, 950 Main Street, 01610, Worcester, MA, USA.
Dhaval NanavatiGlobal Protein Sciences, AbbVie Bioresearch Center, 100 Research Dr, 01605, Worcester, MA, USA.
Samir DasCarlson School of Chemistry and Biochemistry, Clark University, 950 Main Street, 01610, Worcester, MA, USA.
Arundhati NagCarlson School of Chemistry and Biochemistry, Clark University, 950 Main Street, 01610, Worcester, MA, USA.ORCID 0000-0002-1328-1695

Funding

Development of a novel proximity catalyzed Chemical Epitope Targeting technology for isolating macrocyclic peptide inhibitors of KRas(G12V)-Sos interactionR15GM139155 · NIGMS · CLARK UNIVERSITY (WORCESTER, MA) · PI NAG, ARUNDHATI · 2020 to 2020
$451k
NIGMS NIH HHS R15 GM139155NIGMS NIH HHS R15 GM139155-01
6 · The paper itself

Abstract

While most FDA-approved peptide drugs are cyclic, the robust cyclization chemistry of peptides and the deconvolution of cyclic peptide sequences by using tandem mass spectrometry render cyclic peptide drug discovery difficult. Here we present the successful design of cyclic peptides on solid phase that addresses both of these problems. We demonstrate that this peptide cyclization method using dichloro-s-tetrazine on solid phase allows successful cyclization of a panel of random peptide sequences with various charges and hydrophobicities. The cyclic peptides can be linearized and cleaved from the solid phase by simple UV light irradiation, and we demonstrate that accurate sequence information can be obtained for the UV-cleaved linearized peptides by using tandem mass spectrometry. The tetrazine linker used in the cyclic peptides can further be explored for inverse electron-demand Diels-Alder (IEDDA) reactions for screening or bioconjugation applications in the future.

Indexed as

Heterocyclic CompoundsUltraviolet RaysPeptidesPeptides, CyclicHeterocyclic CompoundsPeptidesPeptides, CyclicbioorthogonalitycyclicpeptidesSnArtetrazine

Identifiers

PMID36471561
PMCPMC10099459

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.