SynthesisParasites & vectors2022
Protocols for Plasmodium gametocyte production in vitro: an integrative review and analysis.
Synthesis in Parasites & vectors, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 17 citations in OpenAlex.
- Pan-African hybridization of PfSPZ increases antigenic diversity and replicative capacity for malaria vaccine design.JCI insight · 2026Article
- The inducible gametocyte producer (iGP1) strain is well-suited to produce both immature and mature gametocytes for subsequent drug sensitivity profiling.Frontiers in cellular and infection microbiology · 2026Article
- Low-volumeMicrobial genomics · 2025Article
- Gametocyte production and transmission fitness of African and AsianAntimicrobial agents and chemotherapy · 2025Article
- Repurposing sulfonamide-scaffolds for enhanced and sustainable malaria therapy.Future medicinal chemistry · 2025Review
- Gametocyte production and transmission fitness of African and AsianbioRxiv : the preprint server for biology · 2025Article
- Induction of gametocytogenesis in human malaria parasites: from stress to genome editing.Frontiers in microbiology · 2025Review
- A human-serum-free medium can induce more infectious P. falciparum gametocytes than a conventional human-serum-containing medium.Scientific reports · 2024Article
- Monitoring the density of3 Biotech · 2023Article
- Novel systems to study vector-pathogen interactions in malaria.Frontiers in cellular and infection microbiology · 2023Review
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 4 countries.
Funding
Abstract
backgroundThe production of Plasmodium gametocytes in vitro is a real challenge. Many protocols have been described, but few have resulted in the production of viable and infectious gametocytes in sufficient quantities to conduct research on-but not limited to-transmission-blocking drug and vaccine development. The aim of this review was to identify and discuss gametocyte production protocols that have been developed over the last two decades.
methodsWe analyzed the original gametocyte production protocols published from 2000 onwards based on a literature search and a thorough review. A systematic review was performed of relevant articles identified in the PubMed, Web of Sciences and ScienceDirect databases.
resultsA total 23 studies on the production of Plasmodium gametocytes were identified, 19 involving in vitro Plasmodium falciparum, one involving Plasmodium knowlesi and three involving ex vivo Plasmodium vivax. Of the in vitro studies, 90% used environmental stressors to trigger gametocytogenesis. Mature gametocytemia of up to 4% was reported.
conclusionsSeveral biological parameters contribute to an optimal production in vitro of viable and infectious mature gametocytes. The knowledge gained from this systematic review on the molecular mechanisms involved in gametocytogenesis enables reproducible gametocyte protocols with transgenic parasite lines to be set up. This review highlights the need for additional gametocyte production protocols for Plasmodium species other than P. falciparum.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.