Evidence map›Paper›PMID 36471277›Full record

ArticleBMC cancer2022

MiR-214-3p targets Ras-related protein 14 (RAB14) to inhibit cellular migration and invasion in esophageal Cancer cells.

Pornima Phatak, Whitney M Burrows, Timothy Michael Creed, Mariam Youssef, Goo Lee, James M Donahue

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC cancer, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.6field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. Effect of miR-214-3p on Cisplatin Resistance in Oral Squamous Cell Carcinoma by Regulating Ferroptosis Through Targeting GPX4.Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology · 2026
    Article
  3. Article
  4. Review
  5. Review
  6. Article
  7. Review
  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 1 country.

Pornima PhatakBirmingham Veterans Affairs Medical Center, Birmingham, AL, USA. pornima.phatak@va.gov.
Whitney M BurrowsDepartment of Surgery, University of Maryland School of Medicine, Baltimore, MD, USA.
Timothy Michael CreedCenter for Stem Cell Biology & Regenerative Medicine, University of Maryland School of Medicine, Baltimore, MD, USA.
Mariam YoussefDepartment of Pathology, University of Alabama at Birmingham, Birmingham, AL, USA.
Goo LeeDepartment of Pathology, University of Alabama at Birmingham, Birmingham, AL, USA.
James M DonahueBirmingham Veterans Affairs Medical Center, Birmingham, AL, USA. jdonahue@uabmc.edu.
University of Alabama at Birmingham · USUniversity of Maryland, Baltimore · USBaltimore VA Medical Center · USBirmingham VA Medical Center · US

Funding

CSRD VA I01 CX001434
6 · The paper itself

Abstract

backgroundMicroRNA (miR)-214-3p is emerging as an important tumor suppressor in esophageal cancer. In this study, we examined the interaction between miR-214-3p and RAB14, a membrane trafficking protein shown to exert oncogenic functions in other malignancies, in esophageal cancer cells.

methodsStudies were performed in a human esophageal epithelial cell line and a panel of esophageal cancer cell lines, as well in human specimens. MiR-214-3p expression was measured by digital PCR. Biotinylated RNA pull-down and luciferase reporter assays assessed binding. The xCELLigence RTCA system measured cell migration and invasion in real time. A lentiviral expression vector was used to create an esophageal cancer cell line stably expressing miR-214-3p.

resultsMiR-214-3p expression was decreased in esophageal cancer cell lines and human specimens compared to non-malignant controls. RAB14 mRNA stability and protein expression were decreased following miR-214-3p overexpression. Binding between miR-214-3p and RAB14 mRNA was observed. Either forced expression of miR-214-3p or RAB14 silencing led to a marked decrease in cellular migration and invasion. Esophageal cancer cells stably expressing miR-214-3p demonstrated decreased growth in a subcutaneous murine model.

conclusionsThese results further support the tumor-suppressive role of miR-214-3p in esophageal cancer cells by demonstrating its ability to regulate RAB14 expression.

Indexed as

Esophageal NeoplasmsMicroRNAsrab GTP-Binding ProteinsAnimalsCell Line, TumorCell MovementCell ProliferationGene Expression Regulation, NeoplasticGenes, Tumor SuppressorHumansMiceMicroRNAsMIRN214 microRNA, humanRab14 protein, humanrab GTP-Binding ProteinsEsophageal CancerMicro RNARAB14

Identifiers

PMID36471277
PMCPMC9721009
OpenAlexW4310680523

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.