Evidence map›Paper›PMID 36471035›Full record

ArticleThe AAPS journal2022

Stability and Function of Extracellular Vesicles Derived from Immortalized Human Corneal Stromal Stem Cells: A Proof of Concept Study.

Ning Lyu, Robert Knight, Sarah Y T Robertson, Aurelie Dos Santos, Chi Zhang, Chao Ma, Jianjiang Xu, Jie Zheng, Sophie X Deng

Open access · hybridAbstract read
PubMed Publisher
In one paragraph

Article in The AAPS journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed, 1 pooled it
2.0field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 1 synthesis or guideline pooled it, 24 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Ning LyuStein Eye Institute, University of California Los Angeles, Los Angeles, California, USA.
Robert KnightStein Eye Institute, University of California Los Angeles, Los Angeles, California, USA.
Sarah Y T RobertsonStein Eye Institute, University of California Los Angeles, Los Angeles, California, USA.
Aurelie Dos SantosStein Eye Institute, University of California Los Angeles, Los Angeles, California, USA.
Chi ZhangStein Eye Institute, University of California Los Angeles, Los Angeles, California, USA.
Chao MaStein Eye Institute, University of California Los Angeles, Los Angeles, California, USA.
Jianjiang XuEye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University; NHC Key Laboratory of Myopia (Fudan University); Key Laboratory of Myopia, Shanghai Key Laboratory of Visual Impairment and Restoration, Chinese Academy of Medical Sciences, Shanghai, 200031, China.
Jie ZhengStein Eye Institute, University of California Los Angeles, Los Angeles, California, USA.
Sophie X DengStein Eye Institute, University of California Los Angeles, Los Angeles, California, USA. deng@jsei.ucla.edu.
University of California, Los Angeles · USChinese Academy of Medical Sciences & Peking Union Medical College · CN

Funding

Ex Vivo Expansion of Human Limbal Stem Cells for TransplantationR01EY021797 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Sophie Deng · 2012 to 2026
$5.1M
Development of small-molecule Wnt mimetics for corneal epithelial cell regenerationR01EY028557 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI DENG, SOPHIE, ZHENG, JIE J. · 2018 to 2021
$2.0M
NEI NIH HHS R01 EY021797NEI NIH HHS R01 EY028557
6 · The paper itself

Abstract

With significant advancement and development of extracellular vesicle (EV)-based therapies, there is a growing need to understand how their storage affects their physical and functional characteristics. EVs were isolated from the conditioned medium of a corneal stromal stem cell line (imCSSC) using Total Exosome isolation kit (TEI) and ultracentrifugation (UC) combined protocol. Purified EVs were stored at 4°C, - 80°C, room temperature (RT) after lyophilization with or without trehalose for 4 weeks. EVs stored at - 80°C and RT (lyophilization with trehalose) demonstrated a comparable morphology, while the freeze-dried samples without trehalose showed aggregation and degradation under a transmission electron microscope (TEM). Lyophilized samples without trehalose demonstrated a decreased particle concentration, recovery rate and protein concentration, which was remediated by the addition of trehalose. EVs stored at - 80℃ showed no change in the protein expression of CD9, CD63, and CD81. Regardless of the storage condition, all EV samples investigated reduced inflammation, as well as inhibited expression of fibrotic markers in vitro. Lyophilization of EVs with trehalose was a feasible storage method that retained the physical property and in vitro biological activities of EVs after 4 weeks of storage, while - 80°C offered the best retention of imCSSC-derived EV physical properties. For the first time, this data demonstrated a practical and translatable method for the storage of CSSC-derived EVs for clinical use.

Indexed as

Extracellular VesiclesMesenchymal Stem CellsHumansProof of Concept StudyTrehaloseUltracentrifugationTrehaloseCorneal regeneration and diseasesCorneal stromal stem cellsExtracellular vesiclesLyophilization with trehaloseTranslational research

Identifiers

PMID36471035
OpenAlexW4310725938

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.