ArticleeLife2022
Glycolytic flux-signaling controls mouse embryo mesoderm development.
Article in eLife, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 42 citations in OpenAlex.
- Metabolic regulation of oocyte maturation and early embryo development in mammals.Life metabolism · 2026Review
- Article
- A genetically encoded redox bottleneck constrains human developmental rate.bioRxiv : the preprint server for biology · 2026Article
- Inositol biosynthesis is inversely regulated by glycolytic activity.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2026Article
- Patterns of mitochondrial ATP predict tissue folding.Science advances · 2026Article
- Fructose-1,6-bisphosphate couples glycolytic activity to cell adhesion.Nature cell biology · 2026Article
- Metabolic control of feto-placental development and pregnancy outcomes.Nature reviews. Endocrinology · 2026Review
- A noncanonical role of glycolytic metabolites controlling the timing of mouse embryo segmentation.Science advances · 2025Article
- Glycolytic flux controls retinal progenitor cell differentiation via regulating Wnt signaling.eLife · 2025Article
- Glycolytic activity instructs germ layer proportions through regulation of Nodal and Wnt signaling.Cell stem cell · 2025Article
- Zebrafish and cellular models of SELENON-Congenital myopathy exhibit novel embryonic and metabolic phenotypes.Skeletal muscle · 2025Article
- Targeting aldolase A in hepatocellular carcinoma leads to imbalanced glycolysis and energy stress due to uncontrolled FBP accumulation.Nature metabolism · 2025Article
- Metabolic activities are selective modulators for individual segmentation clock processes.Nature communications · 2025Article
- Article
- Cellular and molecular control of vertebrate somitogenesis.Nature reviews. Molecular cell biology · 2024Review
- Mannose controls mesoderm specification and symmetry breaking in mouse gastruloids.Developmental cell · 2024Article
- Historic obstacles and emerging opportunities in the field of developmental metabolism - lessons from Heidelberg.Development (Cambridge, England) · 2024Review
- The stem cell zoo for comparative studies of developmental tempo.Current opinion in genetics & development · 2024Review
- Enhanced glucose metabolism in Tet-deficient mouse embryonic stem cells.Frontiers in epigenetics and epigenomics · 2024Article
- Hitting the Sweet Spot: How Glucose Metabolism Is Orchestrated in Space and Time by Phosphofructokinase-1.Cancers · 2023Review
Corrections and comments
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Authors and funding
9 authors at 7 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
How cellular metabolic state impacts cellular programs is a fundamental, unresolved question. Here, we investigated how glycolytic flux impacts embryonic development, using presomitic mesoderm (PSM) patterning as the experimental model. First, we identified fructose 1,6-bisphosphate (FBP) as an in vivo sentinel metabolite that mirrors glycolytic flux within PSM cells of post-implantation mouse embryos. We found that medium-supplementation with FBP, but not with other glycolytic metabolites, such as fructose 6-phosphate and 3-phosphoglycerate, impaired mesoderm segmentation. To genetically manipulate glycolytic flux and FBP levels, we generated a mouse model enabling the conditional overexpression of dominant active, cytoplasmic PFKFB3 (cytoPFKFB3). Overexpression of cytoPFKFB3 indeed led to increased glycolytic flux/FBP levels and caused an impairment of mesoderm segmentation, paralleled by the downregulation of Wnt-signaling, reminiscent of the effects seen upon FBP-supplementation. To probe for mechanisms underlying glycolytic flux-signaling, we performed subcellular proteome analysis and revealed that cytoPFKFB3 overexpression altered subcellular localization of certain proteins, including glycolytic enzymes, in PSM cells. Specifically, we revealed that FBP supplementation caused depletion of Pfkl and Aldoa from the nuclear-soluble fraction. Combined, we propose that FBP functions as a flux-signaling metabolite connecting glycolysis and PSM patterning, potentially through modulating subcellular protein localization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.