Evidence map›Paper›PMID 36469143›Full record

ArticleJournal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry2023

Investigations of cellular copper metabolism in ovarian cancer cells using a ratiometric fluorescent copper dye.

Sumayah Mohammed Asiri, Aviva Levina, Elizabeth J New, Peter A Lay

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Article in Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.8field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Sumayah Mohammed AsiriSchool of Chemistry, The University of Sydney, Sydney, NSW, 2006, Australia.
Aviva LevinaSchool of Chemistry, The University of Sydney, Sydney, NSW, 2006, Australia.
Elizabeth J NewSchool of Chemistry, The University of Sydney, Sydney, NSW, 2006, Australia. elizabeth.new@sydney.edu.au.
Peter A LaySchool of Chemistry, The University of Sydney, Sydney, NSW, 2006, Australia. peter.lay@sydney.edu.au.
University of Sydney · AU

Funding

Australian Research Council DP160104172
6 · The paper itself

Abstract

Imbalances in metal homeostasis have been implicated in the progression and drug response of cancer cells. Understanding these changes will enable identification of new treatment regimes and precision medicine approaches to cancer treatment. In particular, there has been considerable interest in the interplay between copper homeostasis and response to platinum-based chemotherapeutic agents. Here, we have studied differences in the Cu uptake and distributions in the ovarian cancer cell line, A2780, and its cisplatin resistant form, A2780.CisR, by measuring total Cu content and the bioavailable Cu pool. Atomic absorption spectroscopy (AAS) revealed a lower total Cu uptake in A2780.CisR compared to A2780 cells. Conversely, live-cell confocal microscopy studies with the ratiometric Cu(I)-sensitive fluorescent dye, InCCu1, revealed higher relative cellular content of labile Cu in A2780.CisR cells compared with A2780 cells. These results demonstrate that Cu trafficking, homeostasis and speciation are different in the Pt-sensitive and resistant cells and may be associated with the predominance of different phenotypes for A2780 (epithelial) and A2780.CisR (mesenchymal) cells.

Indexed as

Antineoplastic AgentsOvarian NeoplasmsCell Line, TumorCisplatinCopperDrug Resistance, NeoplasmFemaleFluorescent DyesHumansOrganoplatinum CompoundsAntineoplastic AgentsCisplatinCopperFluorescent DyesOrganoplatinum CompoundsAASApoptotic cellsCisplatin resistanceConfocal microscopyCopper homeostasisInCCu1Ovarian cancer

Identifiers

PMID36469143
OpenAlexW4310673736

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.