Evidence map›Paper›PMID 36466657›Full record

ArticleFrontiers in microbiology2022

A PCR-based method for the diagnosis of

Aldo Ummarino, Michele Caputo, Francesco Antonio Tucci, Gaetano Pezzicoli, Ada Piepoli, Annamaria Gentile, Tiziana Latiano, Anna Panza, Nicholas Calà, Antonio Pio Ceglia and 7 more

Open access · goldAbstract read
In one paragraph

Article in Frontiers in microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed, 1 pooled it
2.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 1 synthesis or guideline pooled it, 13 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Fifteen-minute consultation: Threadworm in children.Archives of disease in childhood. Education and practice edition · 2026
    Article
  4. Pinworm (Frontiers in public health · 2026
    Review
  5. Article
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 1 institution in 1 country.

Aldo UmmarinoAgorà Biomedical Sciences, Etromapmacs Pole, Lesina (FG), Italy.
Michele CaputoAgorà Biomedical Sciences, Etromapmacs Pole, Lesina (FG), Italy.
Francesco Antonio TucciAgorà Biomedical Sciences, Etromapmacs Pole, Lesina (FG), Italy.
Gaetano PezzicoliAgorà Biomedical Sciences, Etromapmacs Pole, Lesina (FG), Italy.
Ada PiepoliGastroenterology Unit, Fondazione IRCCS "Casa Sollievo Della Sofferenza" Hospital, Viale Cappuccini, Italy.
Annamaria GentileGastroenterology Unit, Fondazione IRCCS "Casa Sollievo Della Sofferenza" Hospital, Viale Cappuccini, Italy.
Tiziana LatianoGastroenterology Unit, Fondazione IRCCS "Casa Sollievo Della Sofferenza" Hospital, Viale Cappuccini, Italy.
Anna PanzaGastroenterology Unit, Fondazione IRCCS "Casa Sollievo Della Sofferenza" Hospital, Viale Cappuccini, Italy.
Nicholas CalàAgorà Biomedical Sciences, Etromapmacs Pole, Lesina (FG), Italy.
Antonio Pio CegliaAgorà Biomedical Sciences, Etromapmacs Pole, Lesina (FG), Italy.
Giovanni PistoioAgorà Biomedical Sciences, Etromapmacs Pole, Lesina (FG), Italy.
Vincenzo TroianoAgorà Biomedical Sciences, Etromapmacs Pole, Lesina (FG), Italy.
Michela PucattiAgorà Biomedical Sciences, Etromapmacs Pole, Lesina (FG), Italy.
Anna LatianoGastroenterology Unit, Fondazione IRCCS "Casa Sollievo Della Sofferenza" Hospital, Viale Cappuccini, Italy.
Angelo AndriulliGastroenterology Unit, Fondazione IRCCS "Casa Sollievo Della Sofferenza" Hospital, Viale Cappuccini, Italy.
Antonio TucciAgorà Biomedical Sciences, Etromapmacs Pole, Lesina (FG), Italy.
Orazio PalmieriGastroenterology Unit, Fondazione IRCCS "Casa Sollievo Della Sofferenza" Hospital, Viale Cappuccini, Italy.
Casa Sollievo della Sofferenza · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Materials and methods: Two subject groups were taken into account: a group of 27 infected patients and a control group of 27 healthy subjects. A nested-PCR was performed on fecal samples to detect Results: Due to the lack of overlap with other organisms, a sequence of the 5S ribosomal DNA (rDNA) spacer region including the tract SL1 was chosen to design appropriate external and internal primers. The first nested-PCR detected Conclusion: The results prove the high diagnostic accuracy of the proposed method, addressing and overcoming the challenges posed by both conventional tests and PCR-based approaches. Therefore, the method can be proposed for clinical application.

Indexed as

Enterobiasis vermicularisPCRpinworm infectionribosomal DNA–rDNAstool (DNA) test

Identifiers

PMID36466657
PMCPMC9712443
OpenAlexW4309614189

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.