Evidence map›Paper›PMID 36463437›Full record

ArticleClinical hemorheology and microcirculation2023

Propofol inhibits cell apoptosis and inflammatory response in ox-LDL-induced human umbilical vein endothelial cells through the modulation of the circ_0003645/miR-149-3p/TRAF7 axis.

Min Chen, Siren Shi, Jianyong Zhao, Qin Pan, Changjun Huang, Quanwei Shen, Zhaohui Liu

RetractedAbstract readRetracted Publication
PubMed Publisher
In one paragraph

Article in Clinical hemorheology and microcirculation, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.3field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Article
  3. Circular RNAs Variously Participate in Coronary Atherogenesis.Current issues in molecular biology · 2023
    Review
  4. The Landscape of Circular RNAs in Cardiovascular Diseases.International journal of molecular sciences · 2023
    Review
4 · The record

Corrections and comments

  • Retraction · 2025-11-23Compromised Peer Review · Concerns/Issues about Referencing/Attributions · Concerns/Issues about Third Party Involvement · Investigation by Journal/Publisher ·
  • Retracted
5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Min ChenDepartment of Anesthesia, First People's Hospital of Linping District, Hangzhou, Zhejiang, China.
Siren ShiDepartment of Anesthesia, First People's Hospital of Linping District, Hangzhou, Zhejiang, China.
Jianyong ZhaoDepartment of Anesthesia, First People's Hospital of Linping District, Hangzhou, Zhejiang, China.
Qin PanDepartment of Anesthesia, First People's Hospital of Linping District, Hangzhou, Zhejiang, China.
Changjun HuangDepartment of Anesthesia, First People's Hospital of Linping District, Hangzhou, Zhejiang, China.
Quanwei ShenDepartment of Anesthesia, First People's Hospital of Linping District, Hangzhou, Zhejiang, China.
Zhaohui LiuDepartment of Anesthesia, First People's Hospital of Linping District, Hangzhou, Zhejiang, China.
Hangzhou First People's Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundPropofol is an anesthetic agent and can impede the progression of human diseases. Circular RNA (circRNA) circ_0003645 has been identified to promote the development of atherosclerosis (AS). This study aimed at the functional mechanism of propofol and circ_0003645 in AS.

methodsAS cell model was established by treatment of oxidized low-density lipoprotein (ox-LDL) in human umbilical vein endothelial cells (HUVECs). Cell viability or apoptosis detection was performed by Cell Counting Kit-8 (CCK-8) assay and flow cytometry. Circ_0003645, microRNA-149-3p (miR-149-3p) and tumor necrosis factor receptor-associated factor 7 (TRAF7) levels were determined by the quantitative real-time polymerase chain reaction (qRT-PCR). Inflammatory cytokines were examined using enzyme-linked immunosorbent assay (ELISA). Protein analysis was conducted by western blot. The interaction of miR-149-3p and circ_0003645 or TRAF7 was analyzed using dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay.

resultsTreatment of ox-LDL inhibited cell viability and enhanced apoptosis in HUVECs to establish the AS cell model. Propofol protected against cell viability inhibition and apoptosis promotion in AS cell model. Circ_0003645 expression was downregulated by propofol in AS cell model. Propofol alleviated cell apoptosis and inflammation by decreasing the circ_0003645 level. Circ_0003645 targeted miR-149-3p, and circ_0003645/miR-149-3p axis was involved in the functional regulation of propofol. TRAF7 was the target of miR-149-3p. Inhibition of miR-149-3p affected the function of propofol by upregulating the TRAF7 expression. Circ_0003645 sponged miR-149-3p to induce the upregulation of TRAF7 following propofol treatment.

conclusionIt has been suggested that propofol acted as an inhibitor against the ox-LDL-induced cell injury by the circ_0003645/miR-149-3p/TRAF7 axis.

Indexed as

AtherosclerosisMicroRNAsPropofolApoptosisCell ProliferationHumansHuman Umbilical Vein Endothelial CellsLipoproteins, LDLTumor Necrosis Factor Receptor-Associated Peptides and ProteinsLipoproteins, LDLMicroRNAsMIRN149 microRNA, humanoxidized low density lipoproteinPropofolTRAF7 protein, humanTumor Necrosis Factor Receptor-Associated Peptides and Proteinsatherosclerosiscirc_0003645miR-149-3pPropofolTRAF7

Identifiers

PMID36463437
OpenAlexW4310282144

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.