ArticleAnalytical and bioanalytical chemistry2023
Highly sensitive and quick in ovo sexing of domestic chicken eggs by two-wavelength fluorescence spectroscopy.
Article in Analytical and bioanalytical chemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Early In Ovo Sex Identification of Chicken Embryos Using a Dual-Stage Difference-Enhanced Spectral Fusion Network Based on Visible-Near-Infrared Transmission Spectroscopy.Animals : an open access journal from MDPI · 2026Article
- Addressing Two Systemic Bottlenecks in Global Poultry Production: A Review of Non-Destructive Pre-Incubation Freshness and In-Ovo Sex Detection.Animals : an open access journal from MDPI · 2026Review
- Nanoparticle-Based Approaches for Enhancing In Vitro Fertilization in Animal Reproduction.International journal of molecular sciences · 2026Review
- Detection of non-invasive sexing of early chick embryos in intact eggs using laser speckle contrast imaging and deep neural networks.PloS one · 2026Article
- Terahertz time domain spectroscopy technique used for evaluation of thickness and optical cum dielectric properties of table and fertile eggshells.Scientific reports · 2025Article
- Non-invasive laser speckle contrast imaging (LSCI) of extra-embryonic blood vessels in intact avian eggs at early developmental stages.Biomedical optics express · 2024Article
- Sustainable animal production: exploring the benefits of sperm sexing technologies in addressing critical industry challenges.Frontiers in veterinary science · 2023Review
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Authors and funding
9 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The in ovo sexing of chicken eggs is a current task and a prerequisite to overcome the mass killing of male day-old chicks from laying lines. Although various methods have been developed and tested in recent years, practicable methods for sex determination are still missing which can be applicated in poultry hatcheries before the chicken embryo is capable of nociception and pain sensation. Optical spectroscopic methods enable an early determination of the sex. In this study, a novel method based on two-wavelength in ovo fluorescence excitation is described. More than 1600 eggs were examined. In ovo fluorescence was sequentially excited at 532 nm and 785 nm. The fluorescence intensities of the spectral regions behave inversely with respect to sex. It is shown that the observed sex-related differences in the fluorescence intensities are based on the embryonic hemoglobin synthesis. The accuracy of sex determination is 96% for both sexes. The hatching rate is not reduced compared to an equivalent reference group.
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