ArticleThe Science of the total environment2023
Multiple traces of monkeypox detected in non-sewered wastewater with sparse sampling from a densely populated metropolitan area in Asia.
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Article in The Science of the total environment, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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17citing papers in PubMed, 1 pooled it
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Serologic evidence of mpox virus exposure among people living with human immunodeficiency virus in Thailand predating clinical recognition during the 2022 outbreak.IJID regions · 2026Article
- Clade-specific multiplex digital PCR assay for monkeypox virus detection in spike-in wastewater samples.Access microbiology · 2026Article
- Wastewater and environmental surveillance as an early warning system and public health tool for mpox outbreak detection and management: a scoping review.BMC medicine · 2025Article
- Review
- Real-time, multi-pathogen wastewater genomic surveillance with Freyja 2.medRxiv : the preprint server for health sciences · 2025Article
- Wastewater Surveillance of Mpox during the Summer Season of 2023 in Slovenia.Infectious disease reports · 2024Article
- Early treatment with fluvoxamine, bromhexine, cyproheptadine, and niclosamide to prevent clinical deterioration in patients with symptomatic COVID-19: a randomized clinical trial.EClinicalMedicine · 2024Article
- Exploring indoor and outdoor dust as a potential tool for detection and monitoring of COVID-19 transmission.iScience · 2024Article
- Enhanced detection of mpox virus in wastewater using a pre-amplification approach: A pilot study informing population-level monitoring of low-titer pathogens.The Science of the total environment · 2023Article
- Monkeypox viral nucleic acids detected using both DNA and RNA extraction workflows.The Science of the total environment · 2023Article
- COVID-19 monitoring with sparse sampling of sewered and non-sewered wastewater in urban and rural communities.iScience · 2023Article
- Monkeypox (Mpox) requires continued surveillance, vaccines, therapeutics and mitigating strategies.Vaccine · 2023Review
- Emergence of monkeypox: a worldwide public health crisis.Human cell · 2023Review
- The study of human monkeypox disease in 2022 using the epidemic models: herd immunity and the basic reproduction number case.Annals of medicine and surgery (2012) · 2023Article
- Socio-demographic determinants of the knowledge and attitude of Nepalese healthcare workers toward human monkeypox: a cross-sectional study.Frontiers in public health · 2023Article
- Environmental detection and spreading of mpox in healthcare settings: a narrative review.Frontiers in microbiology · 2023Review
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Authors and funding
31 authors.
Dhammika Leshan WannigamaDepartment of Infectious Diseases and Infection Control, Yamagata Prefectural Central Hospital, Yamagata, Japan; Department of Microbiology, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand; Center of Excellence in Antimicrobial Resistance and Stewardship, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand; School of Medicine, Faculty of Health and Medical Sciences, The University of Western Australia, Nedlands, Western Australia, Australia; Biofilms and Antimicrobial Resistance Consortium of ODA receiving countries, The University of Sheffield, Sheffield, United Kingdom; Pathogen Hunter's Research Collaborative Team, Department of Infectious Diseases and Infection Control, Yamagata Prefectural Central Hospital, Yamagata, Japan. Electronic address: Dhammika.L@chula.ac.th.
Mohan AmarasiriLaboratory of Environmental Hygiene, Department of Health Science, School of Allied Health Sciences, Graduate School of Medical Sciences, Kitasato University, Kitasato, Sagamihara-Minami, Kanagawa 252-0373, Japan.
Parichart HongsingMae Fah Luang University Hospital, Chiang Rai, Thailand; School of Integrative Medicine, Mae Fah Luang University, Chiang Rai, Thailand.
Cameron HurstMolly Wardaguga Research Centre, Charles Darwin University, Queensland, Australia; Statistics, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
Charin ModchangBiophysics Group, Department of Physics, Faculty of Science, Mahidol University, Bangkok 10400, Thailand; Centre of Excellence in Mathematics, MHESI, Bangkok 10400, Thailand; Thailand Center of Excellence in Physics, Ministry of Higher Education, Science, Research and Innovation, 328 Si Ayutthaya Road, Bangkok 10400, Thailand.
Sudarat ChadsuthiDepartment of Physics, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand.
Suparinthon AnupongBiophysics Group, Department of Physics, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
Phatthranit PhattharapornjaroenFaculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand; Institute of Clinical Sciences, Department of Surgery, Sahlgrenska Academy, Gothenburg University, 40530 Gothenburg, Sweden.
Ali Hosseini Rad S MDepartment of Microbiology and Immunology, University of Otago, Dunedin 9010, Otago, New Zealand; Center of Excellence in Immunology and Immune-Mediated Diseases, Chulalongkorn University, Bangkok 10330, Thailand.
Stefan FernandezDepartment of Virology, U.S. Army Medical Directorate, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand.
Angkana T HuangDepartment of Virology, U.S. Army Medical Directorate, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand.
Naris KueakulpattanaDepartment of Microbiology, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand; Center of Excellence in Antimicrobial Resistance and Stewardship, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Chanikan TanasatitchaiDepartment of Microbiology, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand; Center of Excellence in Antimicrobial Resistance and Stewardship, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Porames VatanaprasanFaculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
Thammakorn SaethangDepartment of Computer Science, Faculty of Science, Kasetsart University, Bangkok, Thailand.
Sirirat Luk-InDepartment of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand.
Robin James StorerOffice of Research Affairs, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Puey OunjaiDepartment of Biology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Naveen Kumar Devanga RagupathiSchool of Medicine, Faculty of Health and Medical Sciences, The University of Western Australia, Nedlands, Western Australia, Australia; Department of Chemical and Biological Engineering, The University of Sheffield, Sheffield, United Kingdom; Department of Clinical Microbiology, Christian Medical College, Vellore, India.
Phitsanuruk KanthaweeSchool of Health Science, Mae Fah Luang University, Thailand.
Daisuke SanoDepartment of Frontier Sciences for Advanced Environment, Graduate School of Environmental Studies, Tohoku University, Sendai, Miyagi, Japan; Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University, Sendai, Miyagi, Japan.
Takashi FurukawaLaboratory of Environmental Hygiene, Department of Health Science, School of Allied Health Sciences, Graduate School of Medical Sciences, Kitasato University, Kitasato, Sagamihara-Minami, Kanagawa 252-0373, Japan.
Kazunari SeiLaboratory of Environmental Hygiene, Department of Health Science, School of Allied Health Sciences, Graduate School of Medical Sciences, Kitasato University, Kitasato, Sagamihara-Minami, Kanagawa 252-0373, Japan.
Asada LeelahavanichkulDepartment of Microbiology, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand; Translational Research in Inflammation and Immunology Research Unit (TRIRU), Department of Microbiology, Chulalongkorn University, Bangkok, Thailand.
Talerngsak KanjanabuchDivision of Nephrology, Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand; Center of Excellence in Kidney Metabolic Disorders, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand; Dialysis Policy and Practice Program (DiP3), School of Global Health, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand; Peritoneal Dialysis Excellence Center, King Chulalongkorn Memorial Hospital, Bangkok, Thailand.
Nattiya HirankarnDepartment of Infectious Diseases and Infection Control, Yamagata Prefectural Central Hospital, Yamagata, Japan; Center of Excellence in Immunology and Immune-Mediated Diseases, Chulalongkorn University, Bangkok 10330, Thailand.
Paul G HigginsInstitute for Medical Microbiology, Immunology and Hygiene, Faculty of Medicine and University Hospital Cologne, University of Cologne, Cologne, Germany; German Centre for Infection Research, Partner site Bonn-Cologne, Cologne, Germany.
Anthony KicicTelethon Kids Institute, University of Western Australia, Nedlands, 6009, Western Australia, Australia; School of Population Health, Curtin University, Bentley 6102, Western Australia, Australia; Centre for Cell Therapy and Regenerative Medicine, Medical School, The University of Western Australia, Nedlands 6009, Western Australia, Australia; Department of Respiratory and Sleep Medicine, Perth Children's Hospital, Nedlands 6009, Western Australia, Australia.
Tanittha ChatsuwanDepartment of Microbiology, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand; Center of Excellence in Antimicrobial Resistance and Stewardship, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. Electronic address: Tanittha.C@chula.ac.th.
Alexander D McLellanDepartment of Microbiology and Immunology, University of Otago, Dunedin 9010, Otago, New Zealand.
Shuichi AbeDepartment of Infectious Diseases and Infection Control, Yamagata Prefectural Central Hospital, Yamagata, Japan; Pathogen Hunter's Research Collaborative Team, Department of Infectious Diseases and Infection Control, Yamagata Prefectural Central Hospital, Yamagata, Japan.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The monkeypox virus is excreted in the feces of infected individuals. Therefore, there is an interest in using viral load detection in wastewater for sentinel early surveillance at a community level and as a complementary approach to syndromic surveillance. We collected wastewater from 63 sewered and non-sewered locations in Bangkok city center between May and August 2022. Monkeypox viral DNA copy numbers were quantified using real-time polymerase chain reaction (PCR) and confirmed positive by Sanger sequencing. Monkeypox viral DNA was first detected in wastewater from the second week of June 2022, with a mean copy number of 16.4 copies/ml (n = 3). From the first week of July, the number of viral DNA copies increased to a mean copy number of 45.92 copies/ml. Positive samples were Sanger sequenced and confirmed the presence of the monkeypox virus. Our study is the first to detect monkeypox viral DNA in wastewater from various locations within Thailand. Results suggest that this could be a complementary source for detecting viral DNA and predicting upcoming outbreaks.
Indexed as
Mpox, MonkeypoxDNA, ViralFecesHumansThailandWastewaterDNA, ViralWastewaterMonkeypoxMonkeypox AsiaMonkeypox viral DNAMonkeypox virus in wastewaterWastewater surveillanceWBE
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