Evidence map›Paper›PMID 36461079›Full record

ArticleActa veterinaria Scandinavica2022

Low-level colonization of methicillin-resistant Staphylococcus aureus in pigs is maintained by slowly evolving, closely related strains in Finnish pig farms.

Marie Verkola, Milla Takala, Suvi Nykäsenoja, Satu Olkkola, Paula Kurittu, Saija Kiljunen, Henni Tuomala, Asko Järvinen, Annamari Heikinheimo

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta veterinaria Scandinavica, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Marie VerkolaDepartment of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 66, 00014 University of Helsinki, Helsinki, Finland. marie.verkola@helsinki.fi.ORCID http://orcid.org/0000-0001-6744-1909
Milla TakalaDepartment of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 66, 00014 University of Helsinki, Helsinki, Finland.
Suvi NykäsenojaLaboratory and Research Division, Microbiology Unit, Finnish Food Authority, P.O. Box 200, 00027 Finnish Food Authority, Helsinki, Finland.
Satu OlkkolaLaboratory and Research Division, Microbiology Unit, Finnish Food Authority, P.O. Box 200, 00027 Finnish Food Authority, Helsinki, Finland.
Paula KurittuDepartment of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 66, 00014 University of Helsinki, Helsinki, Finland.
Saija KiljunenHuman Microbiome Research Program, Faculty of Medicine, Helsinki University Central Hospital, University of Helsinki, P.O. Box 21, 00014 University of Helsinki, Helsinki, Finland.
Henni TuomalaHuman Microbiome Research Program, Faculty of Medicine, Helsinki University Central Hospital, University of Helsinki, P.O. Box 21, 00014 University of Helsinki, Helsinki, Finland.
Asko JärvinenDepartment of Infectious Diseases, Inflammation Center, Helsinki University Central Hospital, University of Helsinki, P.O. Box 340, 00029 HUS, Helsinki, Finland.
Annamari HeikinheimoDepartment of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 66, 00014 University of Helsinki, Helsinki, Finland.
University of Helsinki · FIFinnish Food Authority · FI

Funding

Academy of Finland 304832
6 · The paper itself

Abstract

backgroundOver the past two decades, livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) has become widely prevalent in pig production in Europe. The carriage status of LA-MRSA is known to vary among individual pigs, but bacterial load in pigs has rarely been studied. We assessed the quantity of LA-MRSA in nasal and skin samples of pigs and investigated the genetic diversity of the strains together with sequenced strains from national surveillance and pathology samples from the Finnish Food Authority. On two farms with assumed MRSA-positive status, farm 1 and farm 2, 10 healthy pigs were sampled three times during 2 weeks from the nares and skin (study A). On farm 1, 54 additional pigs were sampled and from confirmed MRSA-positive animals, 10 were randomly selected and transported to a clean, controlled environment for further sampling (study B). From the samples taken on farms 1 and 2 and in the controlled environment, MRSA was isolated both by direct plating and enrichment on selective media. spa types, multilocus sequence types, staphylococcal cassette chromosome mec types, resistance and virulence genes were determined. Core genome multilocus sequence typing (cgMLST) analysis was performed, including the sequences deriving from the surveillance/pathology samples from the Finnish Food Authority.

resultsAll pigs on farm 1 carried LA-MRSA in the nares at all three time points and five pigs on farm 2 at one time point. Nasal quantity varied between 10 and 10

conclusionsThe study shows predominantly low-level carriage (< 10

Indexed as

Methicillin-Resistant Staphylococcus aureusAnimalsFarmsFinlandLivestockSkinSwineAntimicrobial resistancecgMLSTLA-MRSALivestock-associated methicillin-resistant Staphylococcus aureus

Identifiers

PMID36461079
PMCPMC9716547
OpenAlexW4311106765

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.