Evidence map›Paper›PMID 36459293›Full record

ArticleDigestive diseases and sciences2023

Mesenchymal Stem Cells Promote Intestinal Mucosal Repair by Positively Regulating the Nrf2/Keap1/ARE Signaling Pathway in Acute Experimental Colitis.

Peng Liu, Xiao-Ran Xie, Hao Wu, Huan Li, Jing-Shu Chi, Xiao-Ming Liu, Ju Luo, Yu Tang, Can-Xia Xu

Abstract read
PubMed Publisher
In one paragraph

Article in Digestive diseases and sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.9field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 10 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Review
  6. Review
  7. Review
  8. Review
  9. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Peng LiuDepartment of Gastroenterology, Third Xiangya Hospital of Central South University, Changsha, 410013, Hunan, China. liupeng0416@csu.edu.cn.ORCID http://orcid.org/0000-0001-5844-0277
Xiao-Ran XieDepartment of Gastroenterology, Third Xiangya Hospital of Central South University, Changsha, 410013, Hunan, China.
Hao WuDepartment of Gastroenterology, Third Xiangya Hospital of Central South University, Changsha, 410013, Hunan, China.
Huan LiDepartment of Gastroenterology, Third Xiangya Hospital of Central South University, Changsha, 410013, Hunan, China.
Jing-Shu ChiDepartment of Gastroenterology, Third Xiangya Hospital of Central South University, Changsha, 410013, Hunan, China.
Xiao-Ming LiuDepartment of Gastroenterology, Third Xiangya Hospital of Central South University, Changsha, 410013, Hunan, China.
Ju LuoDepartment of Gastroenterology, Third Xiangya Hospital of Central South University, Changsha, 410013, Hunan, China.
Yu TangDepartment of Gastroenterology, Third Xiangya Hospital of Central South University, Changsha, 410013, Hunan, China.
Can-Xia XuDepartment of Gastroenterology, Third Xiangya Hospital of Central South University, Changsha, 410013, Hunan, China.
Central South University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND/

aimsMesenchymal stem cells (MSCs) are a type of adult pluripotent stem cell that has anti-inflammatory and immunomodulatory effects, and whose conditioned medium (CM) has also been found to be effective. We used MSC and CM enemas to investigate their ameliorative effects in a mouse model of colitis.

methodsWe employed MSCs, CM, and MSCs + ML385 (an inhibitor of Nrf2) in dextran sodium sulfate (DSS)-induced colitis. Mice were sacrificed on day 8, and the effects of MSC or CM treatment on the levels of inflammation and oxidative stress in colonic epithelial cells were evaluated by histological analyses.

resultsMSCs inhibited inflammatory cell infiltration and proinflammatory cytokine expression in the colon. In addition, MSCs reduced extracellular matrix deposition and maintained the mechanical barrier and permeability of colonic epithelial cells. Mechanistically, MSCs activated Nrf2, which then increased HO-1 and NQO-1 levels and downregulated the expression of Keap1 to suppress reactive oxygen species production and MDA generation, accompanied by increases in components of the enzymatic antioxidant system, including SOD, CAT, GSH-Px, and T-AOC. However, after administering an Nrf2 inhibitor (ML385) to block the Nrf2/Keap1/ARE pathway, we failed to observe protective effects of MSCs in mice with colitis. CM alone also produced some of the therapeutic benefits of MSCs but was not as effective as MSCs.

conclusionsOur data confirmed that MSCs and CM can effectively improve intestinal mucosal repair in experimental colitis and that MSCs can improve this condition by activating the Nrf2/Keap1/ARE pathway.

Indexed as

ColitisMesenchymal Stem CellsAnimalsDextran SulfateDisease Models, AnimalKelch-Like ECH-Associated Protein 1MiceMice, Inbred C57BLNF-E2-Related Factor 2Signal TransductionDextran SulfateKeap1 protein, mouseKelch-Like ECH-Associated Protein 1NF-E2-Related Factor 2Bone marrow mesenchymal stem cellsNuclear factor erythroid-derived 2-like 2Reactive oxygen speciesUlcerative colitis

Identifiers

PMID36459293
OpenAlexW4310603650

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.