ArticleNature plants2022
The endocytic TPLATE complex internalizes ubiquitinated plasma membrane cargo.
Article in Nature plants, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- Arabidopsis ECHIDNA Plays an Important Role in Auxin Regulation of Clathrin-Mediated Trafficking During Root Gravitropism.Plant, cell & environment · 2026Article
- Direct coupling of nod factor signaling to vesicular trafficking initiates legume nodulation.Nature communications · 2026Article
- Clathrin-Mediated Endocytosis in Plants: Historical to Modern Advances.Traffic (Copenhagen, Denmark) · 2026Review
- Comparative proteomic profiling of receptor kinase signaling reveals key trafficking components enforcing plant stomatal development.Science advances · 2026Article
- Post-Golgi Trafficking in Plant Cells.Sub-cellular biochemistry · 2026Review
- A combined biochemical and computational approach provides evidence for membrane remodelling by the structural scaffold of the endocytic TPLATE complex.Nature plants · 2025Article
- Prevalence and environmental abundance of the TSET complex in cosmopolitan algal groups.iScience · 2025Article
- Brassinosteroid Signaling Dynamics: Ubiquitination-Dependent Regulation of Core Signaling Components.International journal of molecular sciences · 2025Review
- Clathrin-mediated trafficking regulates copper tolerance by modulating the localization of HEAVY METAL ATPase 5 in Arabidopsis root cells.Plant physiology · 2025Article
- Pupylation-based proximity labeling reveals regulatory factors in cellulose biosynthesis in Arabidopsis.Nature communications · 2025Article
- Protein degrons and degradation: Exploring substrate recognition and pathway selection in plants.The Plant cell · 2024Review
- Mechanistic divergences of endocytic clathrin-coated vesicle formation in mammals, yeasts and plants.Journal of cell science · 2024Review
- Biomolecular condensation orchestrates clathrin-mediated endocytosis in plants.Nature cell biology · 2024Article
- Protein Phosphorylation Orchestrates Acclimations of Arabidopsis Plants to Environmental pH.Molecular & cellular proteomics : MCP · 2024Article
Corrections and comments
- Commented on by
- Erratum issued
Authors and funding
15 authors at 3 institutions in 3 countries.
Funding
Abstract
Endocytosis controls the perception of stimuli by modulating protein abundance at the plasma membrane. In plants, clathrin-mediated endocytosis is the most prominent internalization pathway and relies on two multimeric adaptor complexes, the AP-2 and the TPLATE complex (TPC). Ubiquitination is a well-established modification triggering endocytosis of cargo proteins, but how this modification is recognized to initiate the endocytic event remains elusive. Here we show that TASH3, one of the large subunits of TPC, recognizes ubiquitinated cargo at the plasma membrane via its SH3 domain-containing appendage. TASH3 lacking this evolutionary specific appendage modification allows TPC formation but the plants show severely reduced endocytic densities, which correlates with reduced endocytic flux. Moreover, comparative plasma membrane proteomics identified differential accumulation of multiple ubiquitinated cargo proteins for which we confirm altered trafficking. Our findings position TPC as a key player for ubiquitinated cargo internalization, allowing future identification of target proteins under specific stress conditions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.