Evidence map›Paper›PMID 36455857›Full record

ArticleSLAS technology2023

An automated modular open-technology device to measure and adjust concentration of aquatic sperm samples for cryopreservation.

Nikolas Zuchowicz, Yue Liu, W Todd Monroe, Terrence R Tiersch

Abstract read
In one paragraph

Article in SLAS technology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Nikolas ZuchowiczAquatic Germplasm and Genetic Resources Center, School of Renewable Natural Resources, Louisiana State University Agricultural Center, Baton Rouge, LA, USA.
Yue LiuAquatic Germplasm and Genetic Resources Center, School of Renewable Natural Resources, Louisiana State University Agricultural Center, Baton Rouge, LA, USA; Department of Biological and Agricultural Engineering, Louisiana State University & Louisiana State University Agricultural Center, Baton Rouge, LA, USA.
W Todd MonroeDepartment of Biological and Agricultural Engineering, Louisiana State University & Louisiana State University Agricultural Center, Baton Rouge, LA, USA.
Terrence R TierschAquatic Germplasm and Genetic Resources Center, School of Renewable Natural Resources, Louisiana State University Agricultural Center, Baton Rouge, LA, USA. Electronic address: ttiersch@agcenter.lsu.edu.

Funding

Safeguarding Genetic Resources of Aquatic Biomedical Models - Research SupplementalR24OD028443 · OD · LOUISIANA STATE UNIV AGRICULTURAL CENTER · PI Jack Koch, Yue Liu · 2020 to 2026
$5.4M
Development of Germplasm Resources for Preservation of Aquatic ModelsR24OD010441 · OD · LOUISIANA STATE UNIV AGRICULTURAL CENTER · PI BLACKBURN, HARVEY D., MONROE, WILLIAM · 2012 to 2021
$4.3M
NIH HHS R24 OD010441NIH HHS R24 OD028443
6 · The paper itself

Abstract

Repositories for aquatic germplasm are essential for safeguarding valuable genetic diversity for species relevant to aquaculture, biomedical research, and conservation. Development of aquatic germplasm repositories is impeded by a lack of standardization within laboratories and across the research community. Protocols for cryopreservation are often developed ad hoc and without close attention to variables, such as cell concentration, that strongly affect the success and reproducibility of cryopreservation. The wide dissemination and use of specialized tools and devices as open hardware can improve processing reliability and save costs. The goal of the present work was to develop and prototype a modular and open-technology approach to help to standardize the cell concentration of germplasm samples prior to cryopreservation. The specific objectives were to: 1) design and fabricate prototypes of the automated concentration measurement and adjustment system (CMAS), incorporating custom peristaltic pumps and optical evaluation modules, and 2) evaluate the performance of the CMAS with biological samples. Linear regression models were obtained for estimation of aquatic sperm concentration >10

Indexed as

CryopreservationSemenHumansMaleReproducibility of ResultsSpermatozoaTechnology3-D PrintingAquaticConcentrationCryopreservationDilutionOpen technology

Identifiers

PMID36455857
PMCPMC9969519

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.