ArticleJournal of experimental botany2023
Stress response requires an efficient connection between glycogen and central carbon metabolism by phosphoglucomutases in cyanobacteria.
Article in Journal of experimental botany, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 21 citations in OpenAlex.
- Glycogen deficiency impairs diurnal energy metabolism and cell division in Synechocystis.Plant physiology · 2026Article
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- From pond to platform: howJournal of bacteriology · 2026Review
- Enhanced sucrose production by controlling carbon flux through CfrA expression in Synechocystis sp. PCC 6803.Microbial cell factories · 2025Article
- Controlled Carbon Loss: Threshold-Dependent Overflow Metabolism inMicroorganisms · 2025Article
- RAPDOR: Using Jensen-Shannon Distance for the computational analysis of complex proteomics datasets.Nature communications · 2025Article
- Growth Phase-Dependent Changes in the Carbohydrate Metabolism of Penicillium Strains from Diverse Temperature Classes in Response to Cold Stress.International journal of molecular sciences · 2025Article
- Construction of Yeast One-Hybrid Library ofBiomolecules · 2025Article
- Article
- Comparative Morphological, Physiological, and Transcriptomic Analyses of Diploid and Tetraploid Wucai (Plants (Basel, Switzerland) · 2024Article
- Interplay between photosynthetic electron flux and organic carbon sinks in sucrose-excreting Synechocystis sp. PCC 6803 revealed by omics approaches.Microbial cell factories · 2024Article
- Double blocking of carbon metabolism causes a large increase of Calvin-Benson cycle compounds in cyanobacteria.Plant physiology · 2024Article
- Phosphoglucomutase comes into the spotlight.Journal of experimental botany · 2023Article
- Glucose Uncouples Nitrogen Sensing From Chlorosis via a Photosynthetic Checkpoint in Synechocystis sp. PCC 6803.Physiologia plantarumArticle
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4 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Glycogen and starch are the main storage polysaccharides, acting as a source of carbon and energy when necessary. Interconversion of glucose-1-phosphate and glucose-6-phosphate by phosphoglucomutases connects the metabolism of these polysaccharides with central carbon metabolism. However, knowledge about how this connection affects the ability of cells to cope with environmental stresses is still scarce. The cyanobacterium Synechocystis sp. PCC 6803 has two enzymes with phosphoglucomutase activity, PGM (phosphoglucomutase) and PMM/PGM (phosphomannomutase/phosphoglucomutase). In this work, we generated a null mutant of PGM (∆PGM) that exhibits very reduced phosphoglucomutase activity (1% of wild type activity). Although this mutant accumulates moderate amounts of glycogen, its phenotype resembles that of glycogen-less mutants, including high light sensitivity and altered response to nitrogen deprivation. Using an on/off arsenite promoter, we demonstrate that PMM/PGM is essential for growth and responsible for the remaining phosphoglucomutase activity in the ∆PGM strain. Furthermore, overexpression of PMM/PGM in the ∆PGM strain is enough to revoke the phenotype of this mutant. These results emphasize the importance of an adequate flux between glycogen and central carbon metabolism to maintain cellular fitness and indicate that although PGM is the main phosphoglucomutase activity, the phosphoglucomutase activity of PMM/PGM can substitute it when expressed in sufficient amounts.
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