Evidence map›Paper›PMID 36452895›Full record

ArticleFrontiers in medicine2022

Electron microscopy of desmosomal structures in the pemphigus human skin organ culture model.

Uta Katharina Radine, Valéria Bumiller-Bini Hoch, Angelica B Winter Boldt, Detlef Zillikens, Ralf J Ludwig, Christoph M Hammers, Matthias Klinger, Jennifer E Hundt

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in medicine, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.1field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Uta Katharina RadineLübeck Institute of Experimental Dermatology, University of Lübeck, Lübeck, Germany.
Valéria Bumiller-Bini HochLübeck Institute of Experimental Dermatology, University of Lübeck, Lübeck, Germany.
Angelica B Winter BoldtLaboratory of Human Molecular Genetics, Department of Genetics, Federal University of Paraná, Curitiba, Brazil.
Detlef ZillikensDepartment of Dermatology, University of Lübeck, Lübeck, Germany.
Ralf J LudwigLübeck Institute of Experimental Dermatology, University of Lübeck, Lübeck, Germany.
Christoph M HammersLübeck Institute of Experimental Dermatology, University of Lübeck, Lübeck, Germany.
Matthias KlingerInstitute of Anatomy, University of Lübeck, Lübeck, Germany.
Jennifer E HundtLübeck Institute of Experimental Dermatology, University of Lübeck, Lübeck, Germany.
University of Lübeck · DEUniversidade Federal do Paraná · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Pemphigus is a chronic autoimmune skin blistering disease, characterized by acantholysis and by the production of autoantibodies directed against the structural desmosomal proteins desmoglein 1 (DSG1) and/or DSG3. Model systems allow the identification and testing of new therapeutic targets. Here, we evaluated ultrastructural desmosomal morphology in the human skin organ culture (HSOC) model injected with either anti-desmoglein (DSG) 1/3 single-chain variable fragment (scFv, termed Px4-3), Staphylococcus aureus exfoliative toxin (ETA) as a reference and positive control, and normal human IgG as a negative control. Each experimental condition was evaluated in abdominal skin biopsies from five different donors. After 24 h of incubation, we processed the samples for histological and ultrastructural electron microscopy analyses. We found that Px4-3 or ETA induced a loss of desmosomes and increased interdesmosomal widening, similar to patient skin biopsies and other pemphigus models. Thus, we propose the HSOC pemphigus model as an attractive tool to unravel novel therapeutic targets.

Indexed as

desmogleindesmosomeelectron microscopyhuman skin organ culturepemphigus

Identifiers

PMID36452895
PMCPMC9701718
OpenAlexW4309043287

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.