ArticleNature photonics2022
Stain-free identification of cell nuclei using tomographic phase microscopy in flow cytometry.
Article in Nature photonics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 116 citations in OpenAlex.
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- Deep Learning-Guided Holotomography Reveals Early Structural Remodelling During Pluripotency Exit.bioRxiv : the preprint server for biology · 2026Article
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- Plan meta-objective for sub-micron quantitative phase imaging.Light, science & applications · 2026Article
- Three-dimensional forward-scattering imaging flow cytometry system for single-cell analysis.APL photonics · 2025Article
- Virtual Staining-Enabled Colorectal Cancer Metastasis Detection in Liquid Cytology Based on Digital Holography.Sensors (Basel, Switzerland) · 2025Article
- Rolling into the genome: linking mutations to cellular structure through label-free holographic cytometry.Light, science & applications · 2025Article
- Drug-Induced Reversible Lysosomal Changes Tracked in Live Cells by Holo-Tomographic Flow Cytometry.ACS nano · 2025Article
- From genotype to phenotype: decoding mutations in blasts by holo-tomographic flow cytometry.Light, science & applications · 2025Article
- Femtosecond laser microfabrication of a fully-integrated optofluidic device for 3D imaging flow cytometry.Scientific reports · 2025Article
- Neuromorphic-enabled video-activated cell sorting.Nature communications · 2024Article
- High-throughput single-cell sorting by stimulated Raman-activated cell ejection.Science advances · 2024Article
- Chemistry and properties of fluorescent pyrazole derivatives: an approach to bioimaging applications.RSC advances · 2024Review
- Three-dimensional isotropic imaging of live suspension cells enabled by droplet microvortices.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Long-term three-dimensional high-resolution imaging of live unlabeled small intestinal organoids via low-coherence holotomography.Experimental & molecular medicine · 2024Article
- Quantitative phase imaging based on holography: trends and new perspectives.Light, science & applications · 2024Review
- Dynamic label-free analysis of SARS-CoV-2 infection reveals virus-induced subcellular remodeling.Nature communications · 2024Article
- Perspective on quantitative phase imaging to improve precision cancer medicine.Journal of biomedical optics · 2024Article
Corrections and comments
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Authors and funding
14 authors at 4 institutions in 2 countries.
Funding
Abstract
Quantitative Phase Imaging (QPI) has gained popularity in bioimaging because it can avoid the need for cell staining, which in some cases is difficult or impossible. However, as a result, QPI does not provide labelling of various specific intracellular structures. Here we show a novel computational segmentation method based on statistical inference that makes it possible for QPI techniques to identify the cell nucleus. We demonstrate the approach with refractive index tomograms of stain-free cells reconstructed through the tomographic phase microscopy in flow cytometry mode. In particular, by means of numerical simulations and two cancer cell lines, we demonstrate that the nucleus can be accurately distinguished within the stain-free tomograms. We show that our experimental results are consistent with confocal fluorescence microscopy (FM) data and microfluidic cytofluorimeter outputs. This is a significant step towards extracting specific three-dimensional intracellular structures directly from the phase-contrast data in a typical flow cytometry configuration.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.