ArticleAnimal models and experimental medicine2022
Evaluation of direct intramural injection to the bladder wall as a method for developing orthotopic tumor models.
Article in Animal models and experimental medicine, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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2 citing papers in PubMed, 4 citations in OpenAlex.
- Simple procedure for assessing diffuse subarachnoid hemorrhage successfully created using filament perforation method in mice.Animal models and experimental medicine · 2024Article
- Evaluation of direct intramural injection to the bladder wall as a method for developing orthotopic tumor models.Animal models and experimental medicine · 2022Article
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7 authors at 2 institutions in 1 country.
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Abstract
backgroundBladder cancer poses a great burden on society and its high rate of recurrence and treatment failure necessitates use of appropriate animal models to study its pathogenesis and test novel treatments. Orthotopic models are superior to other types since they provide a normal microenvironment. Four methods are described for developing bladder cancer models inside the animal's bladder. Direct intramural injection is one of these methods and is widely used. However, its efficacy in model development has not yet been studied. We aimed to evaluate the efficacy and success rate of the direct intramural injection method of developing an orthotopic model for the study of bladder cancer.
methodTumor cell lines were prepared in four microtubes. Aliquots of 200 × 10
resultsTumors formed in all mice, resulting in 100% take rate and zero post-operation mortality. Surgery time was ≤15 min per mouse. In two mice, tumors were found in the peritoneal space as well.
conclusionDirect intramural injection is a rapid, reliable, and reproducible method for developing orthotopic models of bladder cancer. It can be done on both male and female mice and only requires readily available surgical tools. However, needle track can result in cell spillage and peritoneal tumors.
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