Evidence map›Paper›PMID 36451091›Full record

ArticleBMC genomics2022

Pleiotropic fitness effects of the lncRNA Uhg4 in Drosophila melanogaster.

Rebecca A MacPherson, Vijay Shankar, Lakshmi T Sunkara, Rachel C Hannah, Marion R Campbell, Robert R H Anholt, Trudy F C Mackay

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 83% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 0 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Rebecca A MacPhersonCenter for Human Genetics and Department of Genetics and Biochemistry, Clemson University, 114 Gregor Mendel Circle, Greenwood, SC, 29646, USA.
Vijay ShankarCenter for Human Genetics and Department of Genetics and Biochemistry, Clemson University, 114 Gregor Mendel Circle, Greenwood, SC, 29646, USA.
Lakshmi T SunkaraPresent adress: Clemson Veterinary Diagnostic Center, Livestock Poultry Health, Clemson University, 500 Clemson Road, Columbia, SC, 29229, USA.
Rachel C HannahCenter for Human Genetics and Department of Genetics and Biochemistry, Clemson University, 114 Gregor Mendel Circle, Greenwood, SC, 29646, USA.
Marion R CampbellCenter for Human Genetics and Department of Genetics and Biochemistry, Clemson University, 114 Gregor Mendel Circle, Greenwood, SC, 29646, USA.
Robert R H AnholtCenter for Human Genetics and Department of Genetics and Biochemistry, Clemson University, 114 Gregor Mendel Circle, Greenwood, SC, 29646, USA. ranholt@clemson.edu.ORCID http://orcid.org/0000-0001-7196-8324
Trudy F C MackayCenter for Human Genetics and Department of Genetics and Biochemistry, Clemson University, 114 Gregor Mendel Circle, Greenwood, SC, 29646, USA. tmackay@clemson.edu.
Center for Human Genetics · USClemson University · US

Funding

Statistical Methods for Gene Regulatory Analysis From Single Cell Genomics DataP20GM139769 · NIGMS · CLEMSON UNIVERSITY · PI KONKEL, MIRIAM KRISTINE · 2021 to 2025
$10.8M
Reverse Engineering Quantitative Genetic VariationR01GM128974 · NIGMS · CLEMSON UNIVERSITY · PI ANHOLT, ROBERT R. H, MACKAY, TRUDY F. · 2018 to 2021
$1.9M
Drosophila Models of Rare Mendelian Disorders of Chromatin ModificationF31HD106719 · NICHD · CLEMSON UNIVERSITY · PI MACPHERSON, REBECCA A · 2021 to 2023
$95k
NICHD NIH HHS F31 HD106719NIGMS NIH HHS P20 GM139769NIGMS NIH HHS P20GM139769NIGMS NIH HHS R01 GM128974NIGMS NIH HHS R01GM128974
6 · The paper itself

Abstract

backgroundLong noncoding RNAs (lncRNAs) are a diverse class of RNAs that are critical for gene regulation, DNA repair, and splicing, and have been implicated in development, stress response, and cancer. However, the functions of many lncRNAs remain unknown. In Drosophila melanogaster, U snoRNA host gene 4 (Uhg4) encodes an antisense long noncoding RNA that is host to seven small nucleolar RNAs (snoRNAs). Uhg4 is expressed ubiquitously during development and in all adult tissues, with maximal expression in ovaries; however, it has no annotated function(s).

resultsWe used CRISPR-Cas9 germline gene editing to generate multiple deletions spanning the promoter region and first exon of Uhg4. Females showed arrested egg development and both males and females were sterile. In addition, Uhg4 deletion mutants showed delayed development and decreased viability, and changes in sleep and responses to stress. Whole-genome RNA sequencing of Uhg4 deletion flies and their controls identified co-regulated genes and genetic interaction networks associated with Uhg4. Gene ontology analyses highlighted a broad spectrum of biological processes, including regulation of transcription and translation, morphogenesis, and stress response.

conclusionUhg4 is a lncRNA essential for reproduction with pleiotropic effects on multiple fitness traits.

Indexed as

RNA, Long NoncodingAnimalsDrosophila melanogasterFemaleGene Regulatory NetworksMaleRNA, Small NucleolarRNA SplicingRNA, Long NoncodingRNA, Small NucleolarCRISPRNoncoding RNAOvipositionsnoRNAStress responseSystems genetics

Identifiers

PMID36451091
PMCPMC9710044
OpenAlexW4310596893

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.