ReviewNature protocols2023
Growing and making nano- and microcrystals.
Review in Nature protocols, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- Quantifying optical scattering losses in pump-probe serial femtosecond crystallography experiments.IUCrJ · 2026Article
- A user-friendly goniometer-compatible fixed-target platform for macromolecular crystallography at synchrotrons.Journal of applied crystallography · 2026Article
- Automated microbatch-under-oil phase diagrams to rationalize serial crystallography sample preparation.IUCrJ · 2026Article
- Integrated structural dynamics uncover a new BNature · 2026Article
- Assessing the problem of excitation light scattering in high-viscosity jets used in serial crystallography sample delivery.Journal of applied crystallography · 2025Article
- Sample delivery methods for protein X-ray crystallography with a special focus on sample consumption.Nature communications · 2025Review
- Preparing for successful protein crystallization experiments.Acta crystallographica. Section F, Structural biology communications · 2025Article
- Advances in microfluidic mixers for time-resolved structural biology with X-rays.Biophysical reviews · 2025Review
- Review
- A Rapid and Surfactant-Free Synthesis Strategy for Variously Faceted Cuprous Oxide Polyhedra.Nanomaterials (Basel, Switzerland) · 2025Article
- Sheet-on-sheet fixed target data collection devices for serial crystallography at synchrotron and XFEL sources.Journal of applied crystallography · 2024Article
- Article
- Article
- Reaching the potential of electron diffraction.Cell reports. Physical science · 2024Article
- Applying 3D ED/MicroED workflows toward the next frontiers.Acta crystallographica. Section C, Structural chemistry · 2024Article
- Article
- Article
- All polymer microfluidic chips-A fixed target sample delivery workhorse for serial crystallography.Biomicrofluidics · 2023Article
- Room temperature crystallography and X-ray spectroscopy of metalloenzymes.Methods in enzymology · 2023Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Thanks to recent technological advances in X-ray and micro-electron diffraction and solid-state NMR, structural information can be obtained by using much smaller crystals. Thus, microcrystals have become a valuable commodity rather than a mere stepping stone toward obtaining macroscopic crystals. Microcrystals are particularly useful for structure determination using serial data collection approaches at synchrotrons and X-ray free-electron lasers. The latter's enormous peak brilliance and short X-ray pulse duration mean that structural information can be obtained before the effects of radiation damage are seen; these properties also facilitate time-resolved crystallography. To establish defined reaction initiation conditions, microcrystals with a desired and narrow size distribution are critical. Here, we describe milling and seeding techniques as well as filtration approaches for the reproducible and size-adjustable preparation of homogeneous nano- and microcrystals. Nanocrystals and crystal seeds can be obtained by milling using zirconium beads and the BeadBug homogenizer; fragmentation of large crystals yields micro- or nanocrystals by flowing crystals through stainless steel filters by using an HPLC pump. The approaches can be scaled to generate micro- to milliliter quantities of microcrystals, starting from macroscopic crystals. The procedure typically takes 3-5 d, including the time required to grow the microcrystals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.