Evidence map›Paper›PMID 36450878›Full record

ArticleScientific reports2022

Rapid identification of Staphylococcus aureus based on a fluorescence imaging/detection platform that combines loop mediated isothermal amplification assay and the smartphone-based system.

Patricia Cabrales-Arellano, Edward Park, Martha Minor, Efren Delgado, Delia Valles-Rosales, Heidi Taboada, José Espiritu, Jianzhong Su, Young Ho Park

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Patricia Cabrales-ArellanoFamily and Consumer Science Department, New Mexico State University, Las Cruces, NM, 88003, USA.
Edward ParkBiology Department, New Mexico State University, Las Cruces, NM, 88003, USA.
Martha MinorFamily and Consumer Science Department, New Mexico State University, Las Cruces, NM, 88003, USA.
Efren DelgadoFamily and Consumer Science Department, New Mexico State University, Las Cruces, NM, 88003, USA.
Delia Valles-RosalesIndustrial Management and Technology Department, Texas A&M University Kingsville, Kingsville, TX, 78363, USA.
Heidi TaboadaMechanical and Industrial Engineering Department, Texas A&M University Kingsville, Kingsville, TX, 78363, USA.
José EspirituMechanical and Industrial Engineering Department, Texas A&M University Kingsville, Kingsville, TX, 78363, USA.
Jianzhong SuDepartment of Mathematics, University of Texas at Arlington, Arlington, TX, 76019, USA.
Young Ho ParkMechanical Engineering Department, New Mexico State University, Las Cruces, NM, 88003, USA. ypark@nmsu.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Food associated diseases pose significant public health threat in the United States. Health risks associated with food-borne pathogens drive the need for constant monitoring of food products. An efficient method that can diagnose food-borne pathogens rapidly will be invaluable and in high demand. In this study, we showed the feasibility of a novel rapid detection platform based on fluorescence imaging/detection that combines a user-friendly, portable loop mediated isothermal amplification (LAMP) reaction device and a smartphone-based detection system. The proposed platform was used to detect Staphylococcus aureus which is one of the most important food-borne pathogen especially dairy products. The complete protocol is quicker; the reaction is performed under isothermal conditions and completed in 1 h or less. Experimental results show that LAMP assays were ten-fold more sensitive than PCR-based detection. The proposed smartphone detection system was able to detect and quantify LAMP assay samples containing three different concentrations of S. aureus from 10

Indexed as

Staphylococcal InfectionsStaphylococcus aureusHumansMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesOptical ImagingSmartphone

Identifiers

PMID36450878
PMCPMC9712598

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.